Detailed information of TA system TA115645

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 938459..939193
• Replicon: chromosome
• Accession: NZ_CP034276
• Organism: Pectobacterium versatile strain 14A

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: EIP93_RS04320
• Protein ID: WP_125232324.1
• Coordinates: 938459..938788 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A7V8ILV3
• Locus tag: EIP93_RS04325
• Protein ID: WP_039345635.1
• Coordinates: 938858..939193 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EIP93_RS04280	933522..934037	-	516	WP_125232317.1	GNAT family N-acetyltransferase	-
EIP93_RS04285	934039..934305	-	267	WP_125232318.1	DUF1778 domain-containing protein	-
EIP93_RS04290	934616..934771	-	156	Protein_822	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	-
EIP93_RS04295	934761..935012	-	252	WP_125232319.1	prevent-host-death protein	-
EIP93_RS04300	935142..935612	+	471	WP_125232320.1	hypothetical protein	-
EIP93_RS04305	935597..935941	+	345	WP_125232321.1	DUF1493 family protein	-
EIP93_RS04310	936395..937045	-	651	WP_125232322.1	Eco29kI family restriction endonuclease	-
EIP93_RS04315	937042..938250	-	1209	WP_125232323.1	DNA cytosine methyltransferase	-
EIP93_RS04320	938459..938788	-	330	WP_125232324.1	TA system toxin CbtA family protein	Toxin
EIP93_RS04325	938858..939193	-	336	WP_039345635.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
EIP93_RS04330	939233..939706	-	474	WP_039345638.1	DNA repair protein RadC	-
EIP93_RS04335	939834..940184	-	351	WP_125232325.1	hypothetical protein	-
EIP93_RS04340	940241..941074	-	834	WP_125232326.1	hypothetical protein	-
EIP93_RS22720	941221..941379	-	159	WP_154715508.1	hypothetical protein	-
EIP93_RS04345	941563..942450	-	888	WP_125232327.1	50S ribosome-binding GTPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	933522..952221	18699

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-107)

Antitoxin

• Antitoxin CbeA (7-106)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12627.40 Da
• Isoelectric Point: 4.7627

>T115645 WP_125232324.1 NZ_CP034276:c938788-938459 [Pectobacterium versatile]
MQTTPAILPREDNPCPSPITVWQQLLTYLLEKHYGLTLNDTPFCEENVIQAHIDAGVTLVNAVNFLVEKYELVRIDRNGF
NWQEQSPFRQGIDIYRALRVTGLLTPDWR

Nucleotide

• Length: 330 bp

>T115645 NZ_CP034276:c938788-938459 [Pectobacterium versatile]
ATGCAAACAACACCTGCAATCCTGCCACGGGAGGATAACCCCTGTCCGTCACCTATAACCGTCTGGCAACAACTACTGAC
CTACCTGCTGGAAAAGCACTACGGCCTGACGCTGAACGATACACCATTCTGTGAGGAAAACGTGATTCAGGCGCATATCG
ATGCTGGCGTCACGTTAGTCAATGCCGTCAATTTTCTGGTGGAAAAATACGAACTGGTGCGTATCGATCGCAACGGCTTT
AACTGGCAGGAGCAATCGCCATTTCGTCAGGGGATAGACATTTACCGGGCACTACGAGTAACAGGCCTTCTGACACCTGA
TTGGAGATAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12507.24 Da
• Isoelectric Point: 7.7761

>AT115645 WP_039345635.1 NZ_CP034276:c939193-938858 [Pectobacterium versatile]
MPSSDTPEWGLKCAITPRLGARLVQEGNRLHYLADRASFIGTFSKAEARNLEHVFPELIKQLEQKMRTGELNPRQQGCVT
LHRNEWTCEADTLGSVGYVYIVIYPTSAATR

Nucleotide

• Length: 336 bp

>AT115645 NZ_CP034276:c939193-938858 [Pectobacterium versatile]
ATGCCATCATCGGATACCCCGGAATGGGGACTAAAATGTGCCATCACTCCGCGACTCGGAGCCAGACTGGTGCAGGAAGG
TAACCGTCTGCATTATCTGGCAGACCGGGCCAGCTTTATCGGTACGTTCAGCAAGGCAGAAGCCCGTAATCTGGAACACG
TCTTCCCTGAGCTGATTAAGCAGTTGGAACAGAAAATGCGAACAGGAGAACTGAATCCCCGCCAGCAGGGTTGTGTCACG
TTGCATCGCAACGAATGGACTTGTGAAGCTGATACGCTAGGCAGTGTTGGCTACGTATATATTGTTATTTATCCAACTTC
GGCTGCAACACGCTAA

Structures

Antitoxin

• AlphaFold DB: A0A7V8ILV3