Detailed information of TA system TA115637

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1801536..1802118
• Replicon: chromosome
• Accession: NZ_CP034260
• Organism: Candidatus Desulforudis audaxviator strain BYF

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: B1I5M6
• Locus tag: Daudx_RS08915
• Protein ID: WP_012302900.1
• Coordinates: 1801536..1801892 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: Daudx_RS08920
• Protein ID: WP_200858705.1
• Coordinates: 1801882..1802118 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Daudx_RS08870	1797169..1798083	-	915	WP_012302896.1	putative DNA binding domain-containing protein	-
Daudx_RS08875	1798262..1798642	-	381	WP_012302897.1	hypothetical protein	-
Daudx_RS08880	1798649..1798822	-	174	Protein_1730	DUF1887 domain-containing protein	-
Daudx_RS08885	1798833..1799726	-	894	WP_166485173.1	CRISPR-associated protein Csx14	-
Daudx_RS08890	1799977..1800186	-	210	WP_041570924.1	4Fe-4S binding protein	-
Daudx_RS08895	1800186..1800362	-	177	WP_202034760.1	hypothetical protein	-
Daudx_RS08900	1800487..1800654	-	168	WP_166485175.1	hypothetical protein	-
Daudx_RS08905	1800651..1800980	-	330	WP_012302899.1	helix-turn-helix domain-containing protein	-
Daudx_RS08910	1800970..1801527	-	558	WP_083756654.1	hypothetical protein	-
Daudx_RS08915	1801536..1801892	-	357	WP_012302900.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
Daudx_RS08920	1801882..1802118	-	237	WP_200858705.1	ribbon-helix-helix domain-containing protein	Antitoxin
Daudx_RS08925	1802313..1802789	+	477	WP_012302902.1	hypothetical protein	-
Daudx_RS08930	1802738..1803388	+	651	WP_083756655.1	hypothetical protein	-
Daudx_RS08940	1803723..1803887	-	165	WP_166485176.1	hypothetical protein	-
Daudx_RS08945	1804033..1804974	+	942	WP_012302904.1	hypothetical protein	-
Daudx_RS08950	1804997..1805350	+	354	WP_012302905.1	EscU/YscU/HrcU family type III secretion system export apparatus switch protein	-
Daudx_RS08955	1805347..1805790	+	444	WP_041570926.1	ribonuclease HI	-
Daudx_RS08960	1805875..1806120	-	246	WP_012302907.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-108)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12642.52 Da
• Isoelectric Point: 8.9971

>T115637 WP_012302900.1 NZ_CP034260:c1801892-1801536 [Candidatus Desulforudis audaxviator]
VEIKRGDVFLVNFNPARGSEQAGCRPAVVVQNDVGNKYGPTTIVIAVTTTLKDYPFLVKLSAGEGGLEKDSTANAAQVLT
VDKLRLVRKLGNLTSEKMREINRALAVSLGLTLGETQD

Nucleotide

• Length: 357 bp

>T115637 NZ_CP034260:c1801892-1801536 [Candidatus Desulforudis audaxviator]
GTGGAGATTAAACGTGGCGATGTTTTCCTGGTGAACTTTAACCCGGCGCGGGGAAGTGAGCAGGCGGGCTGTCGGCCCGC
CGTCGTCGTTCAAAACGATGTGGGCAACAAGTATGGCCCAACCACAATTGTGATCGCAGTGACCACTACTCTCAAGGATT
ACCCCTTCCTGGTGAAGTTAAGCGCTGGAGAAGGGGGACTGGAGAAAGACAGCACGGCCAATGCCGCCCAGGTGTTGACC
GTGGACAAGTTGCGCTTGGTGCGCAAGCTGGGGAATCTCACTTCAGAGAAAATGCGCGAGATAAATCGGGCGCTGGCGGT
GAGCCTTGGGTTGACTCTCGGAGAGACCCAGGATTAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8713.96 Da
• Isoelectric Point: 5.6957

>AT115637 WP_200858705.1 NZ_CP034260:c1802118-1801882 [Candidatus Desulforudis audaxviator]
VNAKIAVSLPEQLLSVLDSLARKWATTRSGAVAELLRRAEQEELEVRLRQGYLEMAELHKAHAELFLPSQAEVFRGGD

Nucleotide

• Length: 237 bp

>AT115637 NZ_CP034260:c1802118-1801882 [Candidatus Desulforudis audaxviator]
GTGAACGCGAAAATCGCCGTAAGTTTGCCGGAACAGCTTCTCTCCGTTTTGGATTCCTTGGCCCGGAAATGGGCCACCAC
CAGAAGCGGCGCTGTAGCCGAGCTGCTGCGGCGTGCGGAGCAGGAGGAGCTGGAGGTCCGGCTCAGGCAAGGCTATCTTG
AAATGGCGGAGCTTCACAAAGCCCACGCCGAGCTTTTCCTTCCGTCGCAAGCAGAGGTGTTTCGCGGTGGAGATTAA

Structures

Toxin

• AlphaFold DB: B1I5M6