Detailed information of TA system TA115584

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 1667922..1668420
• Replicon: chromosome
• Accession: NZ_CP034240
• Organism: Aliivibrio salmonicida strain VS224

Toxin (Protein)

• Gene name: -
• Uniprot ID: B6EME2
• Locus tag: EIJ81_RS09945
• Protein ID: WP_012550275.1
• Coordinates: 1668157..1668420 (-)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: B6EME1
• Locus tag: EIJ81_RS09940
• Protein ID: WP_012550274.1
• Coordinates: 1667922..1668167 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EIJ81_RS09920	1663827..1665473	-	1647	WP_173362140.1	methyl-accepting chemotaxis protein	-
EIJ81_RS09925	1665707..1666663	+	957	WP_012549047.1	IS30-like element ISVsa7 family transposase	-
EIJ81_RS09930	1666858..1667133	+	276	WP_044583260.1	DUF1778 domain-containing protein	-
EIJ81_RS09935	1667130..1667633	+	504	WP_012550273.1	GNAT family N-acetyltransferase	-
EIJ81_RS09940	1667922..1668167	-	246	WP_012550274.1	hypothetical protein	Antitoxin
EIJ81_RS09945	1668157..1668420	-	264	WP_012550275.1	BrnT family toxin	Toxin
EIJ81_RS09950	1668600..1669718	-	1119	WP_012550276.1	hypothetical protein	-
EIJ81_RS09955	1669711..1670019	-	309	WP_012550277.1	hypothetical protein	-
EIJ81_RS09960	1670001..1670663	-	663	WP_012550278.1	hypothetical protein	-
EIJ81_RS09965	1670844..1671158	-	315	WP_012550279.1	type II toxin-antitoxin system MqsA family antitoxin	-
EIJ81_RS09970	1671145..1671477	-	333	WP_023603298.1	type II toxin-antitoxin system RelE/ParE family toxin	-
EIJ81_RS09975	1671686..1672135	-	450	WP_044583261.1	hypothetical protein	-
EIJ81_RS09980	1672300..1672755	-	456	WP_012550282.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (6-81)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10100.47 Da
• Isoelectric Point: 6.7246

>T115584 WP_012550275.1 NZ_CP034240:c1668420-1668157 [Aliivibrio salmonicida]
MVNFEFDEVKSSSNLEKHGVDFNVAQLLWNDPDLIEIPVNTHDEPRYVVIAMLNGKHWTGVITYRGQNIRIISVRRSRKG
EVKVYES

Nucleotide

• Length: 264 bp

>T115584 NZ_CP034240:c1668420-1668157 [Aliivibrio salmonicida]
ATGGTTAATTTTGAATTTGATGAAGTTAAGAGTAGCTCGAATCTTGAAAAACATGGTGTGGATTTCAATGTAGCTCAATT
GCTTTGGAATGACCCAGATTTAATCGAGATCCCAGTAAATACTCACGATGAACCAAGATATGTCGTAATCGCAATGCTTA
ATGGTAAACATTGGACAGGCGTTATTACATATCGAGGTCAAAATATCCGAATAATTTCTGTTCGACGCTCAAGAAAAGGT
GAGGTAAAGGTTTATGAAAGCTAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9244.40 Da
• Isoelectric Point: 5.6845

>AT115584 WP_012550274.1 NZ_CP034240:c1668167-1667922 [Aliivibrio salmonicida]
MKANEFDAKFDDGDDILGDLDLSKSKRPMQSQKRVNVDFPTWMIESLDREANRIGVTRQSIIKVWLAERLESVAANHTAS
H

Nucleotide

• Length: 246 bp

>AT115584 NZ_CP034240:c1668167-1667922 [Aliivibrio salmonicida]
ATGAAAGCTAATGAATTTGATGCAAAATTTGACGATGGCGACGATATTCTAGGCGATTTAGATTTATCTAAATCTAAGCG
TCCAATGCAAAGTCAAAAACGAGTAAATGTTGATTTTCCAACATGGATGATCGAATCATTAGATCGTGAAGCGAATCGTA
TAGGTGTAACTCGCCAATCGATTATTAAAGTTTGGTTAGCGGAAAGGTTAGAAAGTGTAGCGGCGAATCACACAGCAAGT
CACTGA

Structures

Toxin

• AlphaFold DB: B6EME2

Antitoxin

• AlphaFold DB: B6EME1