Detailed information of TA system TA115577

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 3463349..3464012
• Replicon: chromosome
• Accession: NZ_CP034237
• Organism: Pectobacterium carotovorum subsp. carotovorum strain JR1.1

Toxin (Protein)

• Gene name: tad
• Uniprot ID: -
• Locus tag: EH204_RS15490
• Protein ID: WP_103940843.1
• Coordinates: 3463349..3463693 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: -
• Locus tag: EH204_RS15495
• Protein ID: WP_161509658.1
• Coordinates: 3463695..3464012 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EH204_RS15470	3460146..3460454	-	309	WP_161509654.1	hypothetical protein	-
EH204_RS15475	3460495..3461115	-	621	WP_161509655.1	FMN-dependent NADH-azoreductase	-
EH204_RS15480	3461150..3461923	-	774	WP_161509656.1	alpha/beta hydrolase	-
EH204_RS15485	3462089..3463117	+	1029	WP_161509657.1	helix-turn-helix domain-containing protein	-
EH204_RS15490	3463349..3463693	+	345	WP_103940843.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EH204_RS15495	3463695..3464012	+	318	WP_161509658.1	helix-turn-helix domain-containing protein	Antitoxin
EH204_RS15500	3464226..3464441	-	216	WP_161509659.1	helix-turn-helix domain-containing protein	-
EH204_RS15505	3464793..3465110	-	318	WP_029368766.1	hypothetical protein	-
EH204_RS15510	3465127..3465441	-	315	WP_161509660.1	hypothetical protein	-
EH204_RS15515	3466219..3467295	-	1077	WP_161509661.1	RES family NAD+ phosphorylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3456621..3467295	10674

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (34-86)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12418.13 Da
• Isoelectric Point: 9.7825

>T115577 WP_103940843.1 NZ_CP034237:3463349-3463693 [Pectobacterium carotovorum subsp. carotovorum]
MKSLYWVGSSKKDLQSLPADVQDVFGYALHLAQTGGKHTQAKPLKGFGGAGVLEVVEDYIGDTYRAVYTVKFGEAVYVLH
AFQKKSSSGIATQKPDMNTIRERLKTAENHAKGA

Nucleotide

• Length: 345 bp

>T115577 NZ_CP034237:3463349-3463693 [Pectobacterium carotovorum subsp. carotovorum]
ATGAAATCACTCTACTGGGTTGGCAGTAGCAAAAAGGATCTCCAGTCGCTACCTGCAGATGTGCAGGATGTGTTCGGTTA
TGCACTTCATCTGGCTCAAACTGGTGGTAAACACACTCAAGCTAAGCCGCTGAAAGGTTTTGGTGGTGCGGGCGTATTAG
AAGTCGTTGAGGATTATATCGGCGATACTTATCGCGCAGTCTACACCGTCAAATTTGGAGAAGCAGTATACGTTCTACAT
GCTTTTCAGAAAAAATCGTCATCTGGAATTGCAACACAGAAACCCGATATGAACACCATTCGTGAACGCCTTAAAACCGC
AGAGAATCATGCAAAAGGAGCATAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11387.42 Da
• Isoelectric Point: 10.4050

>AT115577 WP_161509658.1 NZ_CP034237:3463695-3464012 [Pectobacterium carotovorum subsp. carotovorum]
MSQDIEVSSGNVYADLGNLHAEDMLVKAQLATLISSIIKSLRLTQAEAAKLLGIPQPKLSNLLRGQFRGISETKMLECLT
KLGRDIQIVVGKPRRTPGSVKVVFA

Nucleotide

• Length: 318 bp

>AT115577 NZ_CP034237:3463695-3464012 [Pectobacterium carotovorum subsp. carotovorum]
ATGAGCCAGGATATTGAAGTCAGCAGCGGTAATGTATACGCCGACCTTGGTAACTTGCATGCTGAAGATATGTTGGTTAA
AGCGCAGCTTGCCACCCTGATTAGCTCAATCATCAAAAGCCTCCGTCTCACACAGGCAGAAGCAGCGAAGCTATTGGGCA
TACCCCAACCAAAGCTATCCAACCTATTGCGTGGCCAATTCAGGGGAATCAGCGAAACCAAAATGCTGGAATGCCTTACG
AAGTTGGGACGCGATATACAGATCGTTGTAGGGAAGCCACGCCGCACTCCGGGCAGCGTTAAAGTCGTGTTTGCATGA