Detailed information of TA system TA115576

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 2989248..2989801
• Replicon: chromosome
• Accession: NZ_CP034237
• Organism: Pectobacterium carotovorum subsp. carotovorum strain JR1.1

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: EH204_RS13355
• Protein ID: WP_161509494.1
• Coordinates: 2989248..2989562 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: -
• Locus tag: EH204_RS13360
• Protein ID: WP_161509495.1
• Coordinates: 2989565..2989801 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EH204_RS13335	2984588..2985898	-	1311	WP_189645171.1	site-specific integrase	-
EH204_RS13340	2985900..2987105	-	1206	WP_010305236.1	site-specific integrase	-
EH204_RS13345	2987388..2987545	-	158	Protein_2612	AlpA family phage regulatory protein	-
EH204_RS13350	2987668..2988522	-	855	WP_181919152.1	hypothetical protein	-
EH204_RS13355	2989248..2989562	-	315	WP_161509494.1	CcdB family protein	Toxin
EH204_RS13360	2989565..2989801	-	237	WP_161509495.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
EH204_RS13365	2989944..2990396	-	453	Protein_2616	ArdC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (9-78)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11576.53 Da
• Isoelectric Point: 7.9859

>T115576 WP_161509494.1 NZ_CP034237:c2989562-2989248 [Pectobacterium carotovorum subsp. carotovorum]
MQFTVYGNTGKSVVYPLLLDVTSDIIGQLNRRIVIPLLPIEKYPAGRRPERLVPLVRLTDGKEYAVMTHELASIPVQALG
AVFCDASQYRNQVKAAIDFLIDGF

Nucleotide

• Length: 315 bp

>T115576 NZ_CP034237:c2989562-2989248 [Pectobacterium carotovorum subsp. carotovorum]
ATGCAATTCACTGTATACGGTAATACCGGGAAAAGCGTCGTTTACCCGCTGTTGCTCGATGTTACGAGCGATATTATTGG
ACAATTGAATCGTCGGATAGTGATCCCATTACTCCCGATTGAAAAGTATCCGGCAGGCCGCCGACCGGAGCGCCTTGTCC
CCCTCGTCAGGCTGACGGACGGCAAAGAATACGCCGTAATGACCCACGAGCTGGCAAGTATCCCTGTCCAGGCCCTGGGT
GCGGTGTTTTGTGATGCGTCGCAGTACCGTAATCAGGTAAAGGCGGCAATAGACTTCCTCATCGACGGGTTCTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8955.03 Da
• Isoelectric Point: 7.1670

>AT115576 WP_161509495.1 NZ_CP034237:c2989801-2989565 [Pectobacterium carotovorum subsp. carotovorum]
MTAKQRNTQSVTMTVERALLVRAREAGINLSATLSTALDAELRRHEAKKWQEENKEALAALNRFHDEQGCFSDEYRTF

Nucleotide

• Length: 237 bp

>AT115576 NZ_CP034237:c2989801-2989565 [Pectobacterium carotovorum subsp. carotovorum]
ATGACCGCGAAACAACGCAACACGCAGAGCGTGACCATGACAGTAGAGCGTGCCTTACTGGTAAGAGCGCGTGAAGCGGG
CATTAATCTGAGTGCTACTCTGTCAACCGCATTGGATGCGGAGCTTCGCCGTCATGAAGCGAAAAAATGGCAGGAAGAAA
ACAAGGAAGCTCTCGCAGCACTAAATCGTTTTCATGATGAACAAGGTTGTTTCAGCGATGAATACAGGACGTTTTAA