Detailed information of TA system TA115575

Bioinformatically predicted

Overview

TA module

• Type: VII
• Classification (family/domain): HepT-MntA/HepT(toxin)
• Location: 1521551..1522350
• Replicon: chromosome
• Accession: NZ_CP034237
• Organism: Pectobacterium carotovorum subsp. carotovorum strain JR1.1

Toxin (Protein)

• Gene name: hepT
• Uniprot ID: A0A7V8TG63
• Locus tag: EH204_RS06670
• Protein ID: WP_010296752.1
• Coordinates: 1521551..1521970 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: mntA
• Uniprot ID: -
• Locus tag: EH204_RS06675
• Protein ID: WP_161509016.1
• Coordinates: 1521967..1522350 (-)
• Length: 128 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EH204_RS06650	1516661..1517302	+	642	WP_010296746.1	uridine kinase	-
EH204_RS06655	1517410..1517991	+	582	WP_005974037.1	dCTP deaminase	-
EH204_RS06660	1518055..1519890	+	1836	WP_161509015.1	outer membrane assembly protein AsmA	-
EH204_RS06665	1520155..1521507	+	1353	WP_014914716.1	anaerobic C4-dicarboxylate transporter DcuC	-
EH204_RS06670	1521551..1521970	-	420	WP_010296752.1	DUF86 domain-containing protein	Toxin
EH204_RS06675	1521967..1522350	-	384	WP_161509016.1	nucleotidyltransferase domain-containing protein	Antitoxin
EH204_RS06680	1522464..1524050	-	1587	WP_010296756.1	TerC family protein	-
EH204_RS06685	1524800..1525879	+	1080	WP_039506648.1	UDP-N-acetylglucosamine--undecaprenyl-phosphate N-acetylglucosaminephosphotransferase	-
EH204_RS06690	1526005..1527141	+	1137	WP_010296760.1	polysaccharide export protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease HepT-like (13-130)

Antitoxin

• Polymerase beta, nucleotidyltransferase (4-93)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 16052.37 Da
• Isoelectric Point: 6.9682

>T115575 WP_010296752.1 NZ_CP034237:c1521970-1521551 [Pectobacterium carotovorum subsp. carotovorum]
MTDILLNKSSTIQRCLRRIREEFDTEEGFRQNFTKQDSVILNIQRACEAAIDIANYLIRIKQLGIPQSSRDSFALLAANQ
IITIDLSDNLQKMVGLRNIAVHDYQALNLDIVIHVISHRLSDFEHFIKQIARYSDSQHL

Nucleotide

• Length: 420 bp

>T115575 NZ_CP034237:c1521970-1521551 [Pectobacterium carotovorum subsp. carotovorum]
ATGACAGATATCTTGCTCAACAAAAGCAGCACCATACAGAGATGCCTACGTCGTATTCGTGAGGAATTCGATACTGAGGA
AGGGTTTCGTCAAAACTTCACCAAGCAAGATTCCGTCATACTTAACATACAGCGTGCCTGCGAAGCCGCTATTGATATCG
CTAATTATCTTATCCGTATCAAGCAGCTAGGCATACCACAAAGTAGCCGCGATAGCTTCGCGCTTCTTGCTGCAAACCAG
ATTATTACGATTGATCTGTCTGATAATTTGCAAAAAATGGTGGGATTAAGAAATATTGCCGTGCATGACTATCAGGCGCT
CAATCTGGATATTGTCATCCACGTTATTTCTCATCGTTTAAGCGACTTTGAACATTTTATCAAACAAATAGCCCGGTACA
GCGACAGCCAGCACCTTTAG

Antitoxin

Protein

• Length: 128 a.a.
• Molecular weight: 14400.59 Da
• Isoelectric Point: 4.7463

>AT115575 WP_161509016.1 NZ_CP034237:c1522350-1521967 [Pectobacterium carotovorum subsp. carotovorum]
MFDKIVLTLKKQMPDIKIIYLFGSQATGNARADSDIDIAIMATRALDPVERWELSNQLAKEVGHDVDLIDLLQASTVLRM
EIVRNGKLLYDAETAAGEFEMTTLSMYQHLQKERADIIRSFNQDLKA

Nucleotide

• Length: 384 bp

>AT115575 NZ_CP034237:c1522350-1521967 [Pectobacterium carotovorum subsp. carotovorum]
ATGTTCGATAAGATCGTTTTGACACTGAAAAAACAGATGCCCGACATCAAAATTATTTATCTCTTTGGTTCCCAGGCTAC
AGGCAATGCCAGAGCGGACAGCGACATTGATATCGCCATCATGGCTACAAGAGCACTGGATCCCGTTGAACGCTGGGAGC
TATCTAATCAATTAGCAAAGGAAGTGGGTCATGACGTCGATCTTATCGATTTATTGCAGGCTTCAACGGTATTAAGAATG
GAGATCGTTCGTAACGGCAAGCTGCTTTATGATGCGGAAACAGCAGCGGGGGAATTTGAAATGACAACACTTTCAATGTA
CCAGCATCTGCAAAAAGAGCGTGCGGATATCATTCGTAGCTTTAATCAGGATCTAAAAGCATGA

Structures

Toxin

• AlphaFold DB: A0A7V8TG63