Detailed information of TA system TA115574

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1298510..1299146
• Replicon: chromosome
• Accession: NZ_CP034237
• Organism: Pectobacterium carotovorum subsp. carotovorum strain JR1.1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A7V8QAU7
• Locus tag: EH204_RS05645
• Protein ID: WP_039485998.1
• Coordinates: 1298510..1298692 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: EH204_RS05650
• Protein ID: WP_095698792.1
• Coordinates: 1298715..1299146 (+)
• Length: 144 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EH204_RS05620	1293730..1293933	+	204	WP_005976130.1	YbdD/YjiX family protein	-
EH204_RS05625	1293950..1294936	+	987	WP_039506490.1	GTPase	-
EH204_RS05630	1295203..1296585	+	1383	WP_010308764.1	right-handed parallel beta-helix repeat-containing protein	-
EH204_RS05635	1296966..1297445	-	480	WP_039494133.1	Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK	-
EH204_RS05640	1297516..1298334	-	819	WP_161508950.1	TraB/GumN family protein	-
EH204_RS05645	1298510..1298692	+	183	WP_039485998.1	type II toxin-antitoxin system HicA family toxin	Toxin
EH204_RS05650	1298715..1299146	+	432	WP_095698792.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
EH204_RS05655	1299241..1301964	-	2724	WP_161508951.1	copper-exporting P-type ATPase CopA	-
EH204_RS05660	1302081..1302482	+	402	WP_039472426.1	Cu(I)-responsive transcriptional regulator	-
EH204_RS05665	1302538..1302993	-	456	WP_010301274.1	NfeD family protein	-
EH204_RS05670	1302993..1303907	-	915	WP_010301277.1	SPFH/Band 7/PHB domain protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-60)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (6-138)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6539.74 Da
• Isoelectric Point: 10.5014

>T115574 WP_039485998.1 NZ_CP034237:1298510-1298692 [Pectobacterium carotovorum subsp. carotovorum]
MKSTDLIKELAAAGCECKRHNGGSHQIWWSPITGKTFPVPHPKKDLPLGTVKSIKKMAGI

Nucleotide

• Length: 183 bp

>T115574 NZ_CP034237:1298510-1298692 [Pectobacterium carotovorum subsp. carotovorum]
ATGAAATCAACTGACCTGATAAAGGAGCTGGCAGCGGCAGGTTGTGAGTGCAAACGGCATAACGGCGGGAGTCACCAAAT
ATGGTGGTCACCCATTACTGGAAAAACATTTCCAGTGCCGCATCCAAAAAAGGATTTACCACTTGGAACTGTCAAATCCA
TCAAAAAAATGGCGGGGATTTGA

Antitoxin

Protein

• Length: 144 a.a.
• Molecular weight: 16054.99 Da
• Isoelectric Point: 4.3145

>AT115574 WP_095698792.1 NZ_CP034237:1298715-1299146 [Pectobacterium carotovorum subsp. carotovorum]
MFFSVGVETPKDDATAYGLIVPALCTDDYGCFSAADSQQEIARMAREAILTLIDEAVSRGNLDIKRLNDAGHLIYAAMPD
FADYDSWFVLDVDLSAFEGKPQRLNISLPDTLIQRIDNRVREQPGTYRDRSHFLAEAARHELE

Nucleotide

• Length: 432 bp

>AT115574 NZ_CP034237:1298715-1299146 [Pectobacterium carotovorum subsp. carotovorum]
ATGTTTTTTTCAGTCGGTGTTGAAACGCCAAAAGATGATGCTACGGCATATGGGCTGATCGTTCCGGCGTTATGTACTGA
TGATTATGGCTGCTTCTCTGCAGCTGACAGCCAACAAGAGATTGCACGCATGGCGCGGGAAGCGATTTTAACCCTTATTG
ATGAAGCGGTGAGCCGCGGAAATCTGGATATTAAACGACTTAATGACGCTGGACATCTCATCTATGCGGCGATGCCAGAT
TTTGCGGACTACGATAGTTGGTTCGTGCTGGATGTGGATTTATCAGCTTTTGAGGGAAAACCGCAGCGATTGAACATTTC
ACTTCCCGATACGTTGATACAGCGTATTGATAATCGCGTGCGAGAGCAGCCGGGTACTTATCGCGATCGCAGCCATTTTT
TGGCAGAAGCCGCACGCCATGAACTGGAGTAA

Structures

Toxin

• AlphaFold DB: A0A7V8QAU7