Detailed information of TA system TA115572

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 775731..776391
• Replicon: chromosome
• Accession: NZ_CP034237
• Organism: Pectobacterium carotovorum subsp. carotovorum strain JR1.1

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: -
• Locus tag: EH204_RS03410
• Protein ID: WP_072061666.1
• Coordinates: 775978..776391 (+)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: A0A1D7Z1Z6
• Locus tag: EH204_RS03405
• Protein ID: WP_005971623.1
• Coordinates: 775731..775997 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EH204_RS03385	771846..772850	-	1005	WP_039470792.1	LacI family DNA-binding transcriptional regulator	-
EH204_RS03390	772905..773513	-	609	WP_161508816.1	HD domain-containing protein	-
EH204_RS03395	773792..774439	+	648	WP_010301806.1	hemolysin III family protein	-
EH204_RS03400	774494..775495	-	1002	WP_161508817.1	tRNA-modifying protein YgfZ	-
EH204_RS03405	775731..775997	+	267	WP_005971623.1	FAD assembly factor SdhE	Antitoxin
EH204_RS03410	775978..776391	+	414	WP_072061666.1	protein YgfX	Toxin
EH204_RS03415	776460..777395	+	936	WP_161508818.1	23S rRNA (adenine(1618)-N(6))-methyltransferase RlmF	-
EH204_RS03420	777461..777784	-	324	WP_010301820.1	hypothetical protein	-
EH204_RS03425	777813..778331	-	519	WP_039470802.1	flavodoxin FldB	-
EH204_RS03430	778671..779057	-	387	WP_010301824.1	thioesterase family protein	-
EH204_RS03435	779265..780593	-	1329	WP_010301826.1	MHS family MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (2-132)

Antitoxin

• Flavinator of succinate dehydrogenase (8-79)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 16325.29 Da
• Isoelectric Point: 10.7911

>T115572 WP_072061666.1 NZ_CP034237:775978-776391 [Pectobacterium carotovorum subsp. carotovorum]
VAQWRCDLRVSWRMQLLSLVVHGLLVLLILLAPWPDGYAWLWLCLVTMVMFGFIRSQRNIKSRQGEIVLLSETTLNWRQQ
EWQIVKRPWLLKNGILLSLQAVNGKDKQQLWLASDSMGDDEWRHLRQILLQQKHWAR

Nucleotide

• Length: 414 bp

>T115572 NZ_CP034237:775978-776391 [Pectobacterium carotovorum subsp. carotovorum]
GTGGCCCAGTGGCGATGTGACCTTCGTGTTTCCTGGCGCATGCAACTGCTGTCATTGGTTGTGCATGGCCTATTAGTATT
GCTCATTCTGCTGGCACCGTGGCCGGACGGCTACGCGTGGCTATGGCTATGCCTGGTCACGATGGTGATGTTCGGTTTTA
TCCGTAGCCAACGGAATATTAAATCCCGACAGGGGGAAATCGTTCTGCTCAGCGAAACGACCCTGAACTGGCGGCAGCAG
GAATGGCAGATCGTTAAACGACCGTGGCTGCTGAAGAATGGCATATTACTGTCATTACAAGCGGTTAACGGTAAAGACAA
GCAACAGCTGTGGTTGGCATCGGACAGCATGGGCGATGATGAATGGCGTCACTTGCGTCAAATTCTTTTGCAGCAGAAAC
ACTGGGCAAGATAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10507.94 Da
• Isoelectric Point: 5.1392

>AT115572 WP_005971623.1 NZ_CP034237:775731-775997 [Pectobacterium carotovorum subsp. carotovorum]
MEIDNKSRIHWACRRGMRELDIAIMPFFEHDYDTLNDTDKRTFVRLLQSDDPDLFNWLMNHGEPEDGELKRMISLIQTRN
KDRGPVAM

Nucleotide

• Length: 267 bp

>AT115572 NZ_CP034237:775731-775997 [Pectobacterium carotovorum subsp. carotovorum]
ATGGAAATCGATAATAAATCACGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAATTGGATATCGCGATCATGCCGTT
TTTTGAGCATGACTATGACACACTTAATGACACTGATAAGCGCACATTTGTACGCTTGTTACAAAGCGACGATCCCGATT
TATTCAACTGGCTGATGAACCACGGTGAACCGGAGGACGGAGAGCTGAAACGCATGATTTCCCTTATTCAGACGCGAAAT
AAAGACCGTGGCCCAGTGGCGATGTGA

Structures

Antitoxin

• AlphaFold DB: A0A1D7Z1Z6