Detailed information of TA system TA115569

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-higA/HigB-HigA
• Location: 416801..417640
• Replicon: chromosome
• Accession: NZ_CP034237
• Organism: Pectobacterium carotovorum subsp. carotovorum strain JR1.1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: EH204_RS01875
• Protein ID: WP_161508710.1
• Coordinates: 417278..417640 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EH204_RS01870
• Protein ID: WP_039507304.1
• Coordinates: 416801..417274 (-)
• Length: 158 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EH204_RS01855	412114..413037	-	924	WP_161502937.1	aminoimidazole riboside kinase	-
EH204_RS01860	413511..414062	+	552	WP_010297547.1	polymer-forming cytoskeletal protein	-
EH204_RS01865	414094..416811	+	2718	WP_161508709.1	CRISPR-associated helicase Cas3'	-
EH204_RS01870	416801..417274	-	474	WP_039507304.1	helix-turn-helix domain-containing protein	Antitoxin
EH204_RS01875	417278..417640	-	363	WP_161508710.1	type II toxin-antitoxin system HigB family toxin	Toxin
EH204_RS01880	418941..420509	+	1569	WP_161508711.1	type I-E CRISPR-associated protein Cse1/CasA	-
EH204_RS01885	420493..421140	+	648	WP_161508712.1	type I-E CRISPR-associated protein Cse2/CasB	-
EH204_RS01890	421144..422211	+	1068	WP_161508713.1	type I-E CRISPR-associated protein Cas7/Cse4/CasC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	416801..428636	11835

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (73-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (100-155)

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 14161.38 Da
• Isoelectric Point: 9.7305

>T115569 WP_161508710.1 NZ_CP034237:c417640-417278 [Pectobacterium carotovorum subsp. carotovorum]
MRVISAKAITDAMKKHAKWKVGLKLWLDVFDKPSLRFESYEQLCHVWRNTSCWNVDRIPFGLLENESRKGPLDIYIFDIN
GNNCRIIAWLNPNTGSLYVKDVCSHAEYDKWWKAQTKTKR

Nucleotide

• Length: 363 bp

>T115569 NZ_CP034237:c417640-417278 [Pectobacterium carotovorum subsp. carotovorum]
ATGAGAGTCATCTCAGCCAAGGCCATCACCGATGCGATGAAAAAGCATGCGAAATGGAAAGTTGGTTTGAAGTTATGGCT
GGATGTTTTTGACAAGCCATCGCTGCGGTTTGAGTCCTACGAACAGTTATGTCATGTATGGCGAAATACATCTTGCTGGA
ATGTCGATCGCATACCTTTCGGATTGCTAGAAAACGAAAGCCGCAAAGGACCACTTGATATATATATATTTGATATTAAT
GGTAACAATTGCAGAATCATAGCATGGTTAAACCCTAATACGGGTTCTTTGTATGTGAAAGACGTGTGCTCTCACGCAGA
GTACGATAAGTGGTGGAAAGCTCAAACGAAGACAAAAAGGTGA

Antitoxin

Protein

• Length: 158 a.a.
• Molecular weight: 17747.02 Da
• Isoelectric Point: 4.2043

>AT115569 WP_039507304.1 NZ_CP034237:c417274-416801 [Pectobacterium carotovorum subsp. carotovorum]
MRNTSLGSNSSVGISGLIENLPLLLQEASEQLNSLSVLLSACMEPIEDDNDLQERMECINQLFLYSSDVNDIPATFAELI
ADRVYEYEKKHRDVPHVSQAEALAFFIEERGLKQSDLKQIATQSVISDIINGKRTMTLQQVKDFSRYFNVPVETFID

Nucleotide

• Length: 474 bp

>AT115569 NZ_CP034237:c417274-416801 [Pectobacterium carotovorum subsp. carotovorum]
ATGCGAAATACATCTCTGGGTTCTAACAGTTCTGTAGGAATCAGTGGGCTCATCGAAAATTTACCTCTTCTACTTCAGGA
AGCCTCTGAGCAGCTTAACAGCCTGTCTGTTTTACTCTCTGCCTGTATGGAACCTATTGAAGACGATAATGATCTTCAGG
AAAGAATGGAGTGCATCAATCAACTTTTCCTCTATTCCTCTGATGTCAATGATATTCCAGCCACTTTTGCAGAGCTCATC
GCGGACAGAGTCTATGAATATGAAAAGAAACACCGAGATGTGCCCCACGTCAGTCAGGCTGAAGCCTTAGCTTTTTTTAT
TGAAGAAAGAGGGCTGAAACAGTCAGATTTAAAGCAAATTGCAACGCAAAGCGTCATCTCTGACATTATTAACGGTAAGC
GAACCATGACATTACAGCAAGTTAAGGATTTTTCTCGCTACTTTAATGTGCCTGTTGAGACATTTATTGACTGA