Detailed information of TA system TA115566

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 153877..154511
• Replicon: chromosome
• Accession: NZ_CP034237
• Organism: Pectobacterium carotovorum subsp. carotovorum strain JR1.1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: EH204_RS00660
• Protein ID: WP_010308718.1
• Coordinates: 153877..154152 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A221T3Z5
• Locus tag: EH204_RS00665
• Protein ID: WP_010308714.1
• Coordinates: 154149..154511 (+)
• Length: 121 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EH204_RS00645	150896..152266	-	1371	WP_010281575.1	envelope stress sensor histidine kinase CpxA	-
EH204_RS00650	152263..152961	-	699	WP_010308725.1	envelope stress response regulator transcription factor CpxR	-
EH204_RS00655	153115..153639	+	525	WP_039541999.1	cell-envelope stress modulator CpxP	-
EH204_RS00660	153877..154152	+	276	WP_010308718.1	type II toxin-antitoxin system HicA family toxin	Toxin
EH204_RS00665	154149..154511	+	363	WP_010308714.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
EH204_RS00670	154770..158513	+	3744	WP_161508654.1	DUF4132 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-85)

Antitoxin

• Uncharacterised protein family HicB (61-113)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 9997.59 Da
• Isoelectric Point: 11.1875

>T115566 WP_010308718.1 NZ_CP034237:153877-154152 [Pectobacterium carotovorum subsp. carotovorum]
MQGKIASLRKKQRETLSQIFKTPVLSGVKWSSIESLVTALGGEIKEGSGSRVRFLLNGSIARFHRPHPSPDTDKGALVSL
REWLESIGVKP

Nucleotide

• Length: 276 bp

>T115566 NZ_CP034237:153877-154152 [Pectobacterium carotovorum subsp. carotovorum]
ATGCAGGGAAAAATAGCCAGCCTACGAAAGAAGCAGCGGGAAACGCTGAGCCAAATTTTCAAAACACCAGTACTTTCAGG
TGTTAAATGGTCGAGTATTGAGTCACTCGTTACTGCGTTAGGCGGTGAAATTAAGGAAGGTAGCGGGTCACGAGTGCGAT
TTTTATTGAACGGGAGTATCGCCCGATTCCATCGTCCTCATCCGTCACCGGATACCGATAAAGGTGCCCTGGTTAGCCTG
CGTGAATGGCTGGAAAGTATAGGAGTAAAACCATGA

Antitoxin

Protein

• Length: 121 a.a.
• Molecular weight: 13495.29 Da
• Isoelectric Point: 4.6225

>AT115566 WP_010308714.1 NZ_CP034237:154149-154511 [Pectobacterium carotovorum subsp. carotovorum]
MTKALNTPNTMVVSGQPAIISYVPEIGMFRGKFIGLSGYCDFVADSINGLRSEGEISLREYLEDCQENGIEPYEREEKVK
TFTLRYPESFGERLTIAAAERHISVNTFIVETLSEHLKQA

Nucleotide

• Length: 363 bp

>AT115566 NZ_CP034237:154149-154511 [Pectobacterium carotovorum subsp. carotovorum]
ATGACGAAAGCACTCAACACACCAAATACGATGGTTGTTTCTGGACAACCTGCCATTATCAGTTACGTACCGGAAATTGG
TATGTTTCGTGGTAAGTTTATTGGTTTATCTGGCTACTGTGATTTTGTCGCTGACAGTATTAATGGTTTGAGAAGCGAGG
GTGAAATTTCGCTACGCGAATATCTGGAAGACTGTCAGGAAAATGGCATCGAACCTTACGAGCGTGAAGAGAAGGTGAAA
ACATTCACCCTCCGTTACCCTGAGTCATTCGGGGAACGACTGACCATTGCGGCCGCTGAACGTCACATTTCAGTGAATAC
GTTTATTGTTGAAACGCTTAGCGAACACCTGAAACAGGCTTGA

Structures

Antitoxin

• AlphaFold DB: A0A221T3Z5