Detailed information of TA system TA115565

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 4645562..4646169
• Replicon: chromosome
• Accession: NZ_CP034236
• Organism: Pectobacterium carotovorum subsp. carotovorum strain BP201601.1

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A7V8T7U1
• Locus tag: EH203_RS20855
• Protein ID: WP_010300678.1
• Coordinates: 4645780..4646169 (+)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: C6DIE5
• Locus tag: EH203_RS20850
• Protein ID: WP_010300679.1
• Coordinates: 4645562..4645783 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EH203_RS20825	4640943..4641986	+	1044	WP_161502991.1	hypothetical protein	-
EH203_RS20830	4641998..4642636	+	639	WP_161502992.1	hypothetical protein	-
EH203_RS20835	4643142..4643504	-	363	WP_161502993.1	hypothetical protein	-
EH203_RS20840	4643849..4644156	+	308	Protein_4074	hypothetical protein	-
EH203_RS20845	4644399..4645223	+	825	WP_010300683.1	DNA damage-inducible protein D	-
EH203_RS20850	4645562..4645783	+	222	WP_010300679.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
EH203_RS20855	4645780..4646169	+	390	WP_010300678.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
EH203_RS20860	4646512..4647306	+	795	WP_010300676.1	class I SAM-dependent methyltransferase	-
EH203_RS20865	4647585..4648142	+	558	WP_010300674.1	hypothetical protein	-
EH203_RS20870	4648190..4648735	-	546	WP_010300672.1	hypothetical protein	-
EH203_RS20875	4648849..4649235	+	387	WP_010300669.1	hypothetical protein	-
EH203_RS20880	4649332..4649640	+	309	WP_146619640.1	DUF596 domain-containing protein	-
EH203_RS20885	4649651..4650865	-	1215	WP_010300665.1	AI-2E family transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	rfaD	4566560..4654219	87659

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (5-120)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-53)

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 14583.43 Da
• Isoelectric Point: 5.9739

>T115565 WP_010300678.1 NZ_CP034236:4645780-4646169 [Pectobacterium carotovorum subsp. carotovorum]
MNWVSAQEVIAFHDRILQRLPGVAGMPDPGRAEAIIYRVQNRTHYEGITDVFELAATYWVAVARGHIFNDGNKRTAFFVT
MTFLYRNGVLVRDHDNTLENLTVEAATGAKNVDQLAQHLRNLEDEISPT

Nucleotide

• Length: 390 bp

>T115565 NZ_CP034236:4645780-4646169 [Pectobacterium carotovorum subsp. carotovorum]
ATGAACTGGGTTAGCGCACAAGAGGTGATCGCCTTTCATGACCGGATCTTGCAACGTTTGCCCGGCGTAGCGGGAATGCC
CGATCCAGGTCGAGCGGAAGCGATTATCTACCGGGTACAGAATCGTACGCATTATGAAGGGATAACAGACGTCTTCGAAC
TGGCCGCCACTTACTGGGTGGCAGTTGCGCGTGGCCATATTTTTAATGATGGCAATAAGCGTACCGCGTTCTTCGTCACG
ATGACGTTTCTATACCGTAACGGCGTGCTGGTTCGCGATCACGACAACACGTTGGAGAATTTGACGGTAGAGGCTGCAAC
AGGAGCAAAAAACGTTGACCAGTTAGCACAGCATCTACGAAATTTGGAAGATGAGATCAGTCCTACTTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8135.01 Da
• Isoelectric Point: 5.0359

>AT115565 WP_010300679.1 NZ_CP034236:4645562-4645783 [Pectobacterium carotovorum subsp. carotovorum]
MRTYTSTQARANISEVLDAATHGEPVEITRRDGSSAVVISKAEFEAYQNAKLDAEFDLIMQRHGHTIEALTDR

Nucleotide

• Length: 222 bp

>AT115565 NZ_CP034236:4645562-4645783 [Pectobacterium carotovorum subsp. carotovorum]
ATGCGAACCTACACCTCGACTCAAGCCAGAGCCAACATTTCAGAAGTATTAGATGCTGCAACTCACGGCGAGCCGGTCGA
AATTACTCGTCGAGATGGAAGCTCAGCGGTGGTAATCAGCAAAGCGGAATTCGAGGCATACCAGAATGCCAAATTGGACG
CAGAGTTTGATCTGATTATGCAGCGCCACGGCCACACAATCGAAGCGCTGACTGACCGATGA

Structures

Toxin

• AlphaFold DB: A0A7V8T7U1

Antitoxin

• AlphaFold DB: A0A221TFG2