Detailed information of TA system TA115559

Bioinformatically predicted

Overview

TA module

• Type: VII
• Classification (family/domain): HepT-MntA/HepT(toxin)
• Location: 3243855..3244654
• Replicon: chromosome
• Accession: NZ_CP034236
• Organism: Pectobacterium carotovorum subsp. carotovorum strain BP201601.1

Toxin (Protein)

• Gene name: hepT
• Uniprot ID: A0A7V8TG63
• Locus tag: EH203_RS14530
• Protein ID: WP_010296752.1
• Coordinates: 3244235..3244654 (+)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: mntA
• Uniprot ID: -
• Locus tag: EH203_RS14525
• Protein ID: WP_010296754.1
• Coordinates: 3243855..3244238 (+)
• Length: 128 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EH203_RS14510	3239065..3240201	-	1137	WP_010296760.1	polysaccharide export protein	-
EH203_RS14515	3240327..3241406	-	1080	WP_039506648.1	UDP-N-acetylglucosamine--undecaprenyl-phosphate N-acetylglucosaminephosphotransferase	-
EH203_RS14520	3242156..3243742	+	1587	WP_010296756.1	TerC family protein	-
EH203_RS14525	3243855..3244238	+	384	WP_010296754.1	nucleotidyltransferase domain-containing protein	Antitoxin
EH203_RS14530	3244235..3244654	+	420	WP_010296752.1	DUF86 domain-containing protein	Toxin
EH203_RS14535	3244698..3246049	-	1352	Protein_2852	anaerobic C4-dicarboxylate transporter DcuC	-
EH203_RS14540	3246313..3248148	-	1836	WP_010296749.1	outer membrane assembly protein AsmA	-
EH203_RS14545	3248212..3248793	-	582	WP_005974037.1	dCTP deaminase	-
EH203_RS14550	3248901..3249542	-	642	WP_010296746.1	uridine kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease HepT-like (13-130)

Antitoxin

• Polymerase beta, nucleotidyltransferase (4-93)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 16052.37 Da
• Isoelectric Point: 6.9682

>T115559 WP_010296752.1 NZ_CP034236:3244235-3244654 [Pectobacterium carotovorum subsp. carotovorum]
MTDILLNKSSTIQRCLRRIREEFDTEEGFRQNFTKQDSVILNIQRACEAAIDIANYLIRIKQLGIPQSSRDSFALLAANQ
IITIDLSDNLQKMVGLRNIAVHDYQALNLDIVIHVISHRLSDFEHFIKQIARYSDSQHL

Nucleotide

• Length: 420 bp

>T115559 NZ_CP034236:3244235-3244654 [Pectobacterium carotovorum subsp. carotovorum]
ATGACAGATATCTTGCTCAACAAAAGCAGCACCATACAAAGATGCCTGCGTCGTATTCGTGAGGAATTCGATACTGAGGA
AGGGTTTCGTCAAAACTTCACCAAGCAAGATTCCGTCATACTTAACATACAGCGTGCCTGTGAAGCCGCTATTGATATCG
CTAATTATCTTATCCGTATCAAGCAGCTAGGCATACCACAAAGTAGCCGCGATAGCTTCGCGCTTCTTGCTGCAAACCAG
ATTATTACGATTGATCTGTCTGATAATTTGCAAAAAATGGTGGGATTAAGAAATATTGCCGTGCATGACTATCAGGCGCT
CAATCTGGATATTGTCATCCACGTTATTTCTCATCGTTTAAGCGACTTTGAACATTTTATCAAACAAATAGCCCGGTACA
GCGACAGCCAGCACCTTTAG

Antitoxin

Protein

• Length: 128 a.a.
• Molecular weight: 14388.57 Da
• Isoelectric Point: 4.7463

>AT115559 WP_010296754.1 NZ_CP034236:3243855-3244238 [Pectobacterium carotovorum subsp. carotovorum]
MFDKIVLTLKKQMPDIKIIYLFGSQATGNARADSDIDIAIMATRALDPVERWELSNQLAKEVGHDVDLIDLLQASTVLKM
EIVRNGKLLYDSETAAGEFEMTTLSMYQHLQKERADIIRSFNQDLKA

Nucleotide

• Length: 384 bp

>AT115559 NZ_CP034236:3243855-3244238 [Pectobacterium carotovorum subsp. carotovorum]
ATGTTCGATAAGATCGTTTTGACACTGAAAAAACAGATGCCCGACATCAAAATTATTTATCTCTTTGGTTCCCAGGCTAC
AGGCAATGCCAGAGCGGACAGCGACATTGATATCGCCATCATGGCTACAAGAGCACTGGACCCCGTTGAACGCTGGGAGC
TATCTAATCAATTAGCAAAGGAAGTGGGTCATGACGTCGATCTTATCGATTTATTGCAGGCTTCAACGGTATTAAAAATG
GAGATCGTTCGTAACGGCAAGCTGCTTTATGATTCGGAAACAGCAGCGGGGGAATTTGAAATGACAACACTGTCAATGTA
CCAGCATCTGCAAAAAGAGCGTGCGGATATCATTCGTAGCTTTAATCAGGATCTAAAAGCATGA

Structures

Toxin

• AlphaFold DB: A0A7V8TG63