Detailed information of TA system TA115558

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 3011717..3012270
• Replicon: chromosome
• Accession: NZ_CP034236
• Organism: Pectobacterium carotovorum subsp. carotovorum strain BP201601.1

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: EH203_RS13620
• Protein ID: WP_010305012.1
• Coordinates: 3011717..3012031 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: -
• Locus tag: EH203_RS13625
• Protein ID: WP_010305008.1
• Coordinates: 3012034..3012270 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EH203_RS13595	3006775..3007770	+	996	WP_161502630.1	aldo/keto reductase	-
EH203_RS13600	3007880..3008557	-	678	WP_010305027.1	NAD(P)H-dependent oxidoreductase	-
EH203_RS13605	3008790..3009382	-	593	Protein_2666	NAD(P)H-dependent oxidoreductase	-
EH203_RS13610	3009601..3010503	+	903	WP_010305019.1	AraC family transcriptional regulator	-
EH203_RS13615	3011217..3011504	+	288	WP_010305018.1	hypothetical protein	-
EH203_RS13620	3011717..3012031	-	315	WP_010305012.1	CcdB family protein	Toxin
EH203_RS13625	3012034..3012270	-	237	WP_010305008.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
EH203_RS13630	3012413..3012865	-	453	Protein_2671	ArdC family protein	-
EH203_RS13635	3013228..3014121	+	894	Protein_2672	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3013228..3013395	167

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (9-78)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11521.45 Da
• Isoelectric Point: 7.9859

>T115558 WP_010305012.1 NZ_CP034236:c3012031-3011717 [Pectobacterium carotovorum subsp. carotovorum]
MQFTVYGNTGKSVVYPLLLDVTSDIIGQLNRRIVIPLLPIEKYPAGRRPDRLVPVVRLTDGKEYAVMTHELASIPVQALG
AVFCDASQYRSQVKAALDFLIDGF

Nucleotide

• Length: 315 bp

>T115558 NZ_CP034236:c3012031-3011717 [Pectobacterium carotovorum subsp. carotovorum]
ATGCAATTCACTGTATACGGTAATACCGGGAAAAGCGTCGTTTACCCGCTGTTGCTCGATGTCACGAGCGATATTATTGG
GCAATTGAATCGTCGGATAGTGATCCCATTGCTCCCTATTGAAAAGTATCCTGCAGGCCGCCGACCGGATCGCCTTGTCC
CCGTCGTCAGGCTGACGGACGGCAAAGAGTACGCCGTAATGACCCACGAGCTGGCAAGTATCCCTGTCCAGGCCTTGGGG
GCGGTGTTTTGTGATGCTTCGCAGTACCGTTCTCAGGTAAAGGCGGCACTAGACTTCCTCATCGATGGGTTCTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8934.00 Da
• Isoelectric Point: 7.1672

>AT115558 WP_010305008.1 NZ_CP034236:c3012270-3012034 [Pectobacterium carotovorum subsp. carotovorum]
MTAKPRNTQSVTITVERALLVRAREAGINLSATLSTALDAELRRHEAKKWQEENREALAALNRFHDEQGCFSDEYRTF

Nucleotide

• Length: 237 bp

>AT115558 NZ_CP034236:c3012270-3012034 [Pectobacterium carotovorum subsp. carotovorum]
ATGACCGCGAAACCACGCAACACGCAGAGCGTGACCATAACGGTAGAGCGTGCCTTACTGGTAAGAGCGCGTGAAGCGGG
CATCAATCTGAGTGCTACTCTGTCAACCGCATTGGATGCGGAGCTTCGCCGTCATGAAGCGAAAAAATGGCAGGAAGAAA
ACAGGGAGGCTCTCGCAGCACTAAATCGTTTTCATGATGAGCAGGGTTGTTTCAGCGATGAATACAGGACGTTTTAA