Detailed information of TA system TA115552

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 65101..65626
• Replicon: plasmid pATCC14028
• Accession: NZ_CP034231
• Organism: Salmonella enterica subsp. enterica serovar Typhimurium strain ATCC 14028

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: I3W3D5
• Locus tag: EIL76_RS24835
• Protein ID: WP_001159863.1
• Coordinates: 65321..65626 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: M7S5D0
• Locus tag: EIL76_RS24830
• Protein ID: WP_000813641.1
• Coordinates: 65101..65319 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EIL76_RS24800	60388..60769	+	382	Protein_72	YgiW/YdeI family stress tolerance OB fold protein	-
EIL76_RS24805	60938..61382	+	445	Protein_73	site-specific integrase	-
EIL76_RS24810	61641..62630	-	990	WP_001527061.1	RepB family plasmid replication initiator protein	-
EIL76_RS24815	63124..63420	-	297	WP_001687482.1	hypothetical protein	-
EIL76_RS24825	63904..64425	-	522	WP_010999942.1	hypothetical protein	-
EIL76_RS24830	65101..65319	+	219	WP_000813641.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
EIL76_RS24835	65321..65626	+	306	WP_001159863.1	type II toxin-antitoxin system toxin CcdB	Toxin
EIL76_RS24840	65628..65918	+	291	WP_001266176.1	hypothetical protein	-
EIL76_RS24845	65915..66436	+	522	WP_000198608.1	hypothetical protein	-
EIL76_RS24850	66471..67253	+	783	WP_000082169.1	site-specific integrase	-
EIL76_RS24855	67262..67975	+	714	Protein_82	EAL domain-containing protein	-
EIL76_RS24865	67979..68487	+	509	Protein_83	CaiF/GrlA family transcriptional regulator	-
EIL76_RS24870	68481..68966	+	486	WP_000905606.1	membrane protein	-
EIL76_RS24875	69243..69530	-	288	WP_071530243.1	hypothetical protein	-
EIL76_RS24880	69686..70246	+	561	WP_000900095.1	inverse autotransporter beta domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..95924	95924

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 13091.77 Da
• Isoelectric Point: 12.8043

>T115552 WP_001159863.1 NZ_CP034231:65321-65626 [Salmonella enterica subsp. enterica serovar Typhimurium]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK
KKKKKKKKKKKKKKKKKKKKKK

Nucleotide

• Length: 306 bp

>T115552 NZ_CP034231:65321-65626 [Salmonella enterica subsp. enterica serovar Typhimurium]
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 9374.72 Da
• Isoelectric Point: 12.6573

>AT115552 WP_000813641.1 NZ_CP034231:65101-65319 [Salmonella enterica subsp. enterica serovar Typhimurium]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK

Nucleotide

• Length: 219 bp

>AT115552 NZ_CP034231:65101-65319 [Salmonella enterica subsp. enterica serovar Typhimurium]
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA

Structures

Toxin

• AlphaFold DB: I3W3D5

Antitoxin

• AlphaFold DB: A0A656ICA6