Detailed information of TA system TA115549

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 3704502..3705145
• Replicon: chromosome
• Accession: NZ_CP034225
• Organism: Kosakonia sp. CCTCC M2018092

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: EH164_RS17630
• Protein ID: WP_076769435.1
• Coordinates: 3704502..3704918 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: EH164_RS17635
• Protein ID: WP_042715980.1
• Coordinates: 3704915..3705145 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EH164_RS17610	3700585..3702246	-	1662	WP_124971553.1	ShlB/FhaC/HecB family hemolysin secretion/activation protein	-
EH164_RS17620	3703145..3703597	+	453	WP_124971556.1	hypothetical protein	-
EH164_RS17625	3703793..3704407	-	615	WP_124969573.1	hypothetical protein	-
EH164_RS17630	3704502..3704918	-	417	WP_076769435.1	type II toxin-antitoxin system VapC family toxin	Toxin
EH164_RS17635	3704915..3705145	-	231	WP_042715980.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
EH164_RS17645	3705785..3707500	-	1716	WP_124969577.1	AIPR family protein	-
EH164_RS17650	3707748..3707927	-	180	Protein_3429	hypothetical protein	-
EH164_RS17665	3708412..3709665	-	1254	WP_124969579.1	glutamate-5-semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-49)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 14986.30 Da
• Isoelectric Point: 8.5258

>T115549 WP_076769435.1 NZ_CP034225:c3704918-3704502 [Kosakonia sp. CCTCC M2018092]
VNKTYMLDTNICSFIMREQPEAVLKRLEQTVLRNHRIVVSAITYAEMRFGAVGKKASPRHAKLVDAFCARLDAVLAWDRA
AVDATTEIKVALAAAGTPIGPNDTAIAGHAIAAGAILVTNNTREFARVPGLELEDWSR

Nucleotide

• Length: 417 bp

>T115549 NZ_CP034225:c3704918-3704502 [Kosakonia sp. CCTCC M2018092]
GTGAACAAGACTTATATGCTCGACACAAATATTTGCTCATTCATCATGCGCGAGCAGCCTGAGGCAGTCCTGAAACGCCT
GGAGCAGACCGTTCTGCGTAACCACCGCATCGTGGTATCGGCCATCACCTATGCCGAGATGCGCTTTGGTGCTGTCGGCA
AGAAAGCCTCACCGCGTCACGCAAAGCTTGTTGACGCATTCTGCGCCCGTCTTGATGCAGTTCTCGCCTGGGATCGTGCA
GCAGTGGATGCCACGACCGAAATTAAGGTCGCTCTGGCCGCAGCTGGCACACCGATTGGTCCAAATGACACGGCGATTGC
AGGGCACGCCATTGCGGCTGGCGCTATTCTGGTGACCAACAATACCCGTGAGTTTGCCCGCGTTCCCGGACTCGAGCTTG
AAGACTGGAGCCGTTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8767.85 Da
• Isoelectric Point: 4.3654

>AT115549 WP_042715980.1 NZ_CP034225:c3705145-3704915 [Kosakonia sp. CCTCC M2018092]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIIREGDSIILRPVRPTWGSFSQLEKADPDFMAEREDVVTDEGRFDL

Nucleotide

• Length: 231 bp

>AT115549 NZ_CP034225:c3705145-3704915 [Kosakonia sp. CCTCC M2018092]
ATGAGAACCGTATCTATTTTCAAAAACGGCAACAACCGTGCCATACGCCTGCCCCGCGATCTGGATTTTGAAGGGGTGAG
TGAACTGGAGATCATTCGTGAAGGAGACAGCATCATTCTGCGCCCTGTGCGCCCCACTTGGGGCTCTTTCTCTCAACTTG
AGAAGGCCGATCCGGACTTTATGGCGGAGCGTGAGGACGTTGTCACCGATGAAGGACGATTTGATCTGTGA