Detailed information of TA system TA115548

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3510924..3511540
• Replicon: chromosome
• Accession: NZ_CP034225
• Organism: Kosakonia sp. CCTCC M2018092

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: A0A807LE76
• Locus tag: EH164_RS16735
• Protein ID: WP_023478287.1
• Coordinates: 3511322..3511540 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: EH164_RS16730
• Protein ID: WP_023478264.1
• Coordinates: 3510924..3511298 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EH164_RS16720	3506020..3507219	+	1200	WP_124969353.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
EH164_RS16725	3507242..3510391	+	3150	WP_094419552.1	multidrug efflux RND transporter permease subunit AcrB	-
EH164_RS16730	3510924..3511298	+	375	WP_023478264.1	Hha toxicity modulator TomB	Antitoxin
EH164_RS16735	3511322..3511540	+	219	WP_023478287.1	hemolysin expression modulator Hha	Toxin
EH164_RS16740	3511616..3512185	+	570	WP_124969355.1	maltose O-acetyltransferase	-
EH164_RS16745	3512286..3512789	+	504	WP_023478286.1	YlaC family protein	-
EH164_RS16750	3512923..3513624	+	702	WP_124969357.1	GNAT family N-acetyltransferase	-
EH164_RS16755	3513621..3513761	-	141	WP_023478279.1	type B 50S ribosomal protein L36	-
EH164_RS16760	3513765..3514025	-	261	WP_124969359.1	type B 50S ribosomal protein L31	-
EH164_RS16765	3514288..3515316	+	1029	WP_124969361.1	isopenicillin N synthase family oxygenase	-
EH164_RS16770	3515339..3516145	+	807	WP_124969363.1	MetQ/NlpA family ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-115)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8657.04 Da
• Isoelectric Point: 7.9892

>T115548 WP_023478287.1 NZ_CP034225:3511322-3511540 [Kosakonia sp. CCTCC M2018092]
MSEKPLTKIDYLMRLRRCQSIDTLERVIEKNKYELTDDELTVFYSAADHRLAELTMNKLYDKIPSSVWKFVR

Nucleotide

• Length: 219 bp

>T115548 NZ_CP034225:3511322-3511540 [Kosakonia sp. CCTCC M2018092]
ATGTCCGAAAAACCATTAACAAAAATTGATTATTTGATGCGTTTAAGGCGCTGCCAATCAATTGATACGCTTGAACGCGT
TATTGAAAAAAATAAATACGAACTGACCGACGATGAGCTTACCGTGTTTTATTCAGCGGCCGATCATCGTCTGGCTGAAC
TGACCATGAATAAGCTTTATGACAAAATCCCCTCGTCGGTGTGGAAATTTGTTCGCTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14420.22 Da
• Isoelectric Point: 6.0581

>AT115548 WP_023478264.1 NZ_CP034225:3510924-3511298 [Kosakonia sp. CCTCC M2018092]
MDEYSPKRHDIAQLKFLCESLHQECLANLEESHHGWVNDPTSAINLQLNELIEHIATFALNYKIKHDKDNKLIELIDEYL
DDTFMLFSSYGINTHDLQKWRKTGGKLFRSFDNVSKANPVSHSC

Nucleotide

• Length: 375 bp

>AT115548 NZ_CP034225:3510924-3511298 [Kosakonia sp. CCTCC M2018092]
ATGGACGAATACTCGCCGAAAAGGCATGATATTGCGCAGCTTAAATTTCTCTGCGAATCGTTGCACCAGGAGTGCCTCGC
CAATCTGGAAGAGAGCCATCATGGATGGGTTAACGACCCCACATCAGCCATCAACCTGCAGTTAAATGAGCTGATTGAGC
ATATTGCAACCTTCGCGCTGAATTATAAAATTAAGCACGATAAAGATAATAAACTGATTGAATTGATCGACGAATACCTT
GATGACACGTTTATGTTGTTCAGCAGTTACGGTATTAATACACACGACTTACAGAAATGGCGTAAAACAGGCGGTAAGCT
GTTTCGCAGTTTCGACAATGTAAGCAAGGCGAATCCAGTAAGTCACTCTTGTTAA

Structures

Toxin

• AlphaFold DB: A0A807LE76