Detailed information of TA system TA115538

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 791235..791898
• Replicon: chromosome
• Accession: NZ_CP034225
• Organism: Kosakonia sp. CCTCC M2018092

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: -
• Locus tag: EH164_RS03680
• Protein ID: WP_023480882.1
• Coordinates: 791482..791898 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: A0A807LAC0
• Locus tag: EH164_RS03675
• Protein ID: WP_023480982.1
• Coordinates: 791235..791501 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EH164_RS03655	786792..788225	-	1434	WP_124967325.1	6-phospho-beta-glucosidase	-
EH164_RS03660	788342..789070	-	729	WP_124971247.1	MurR/RpiR family transcriptional regulator	-
EH164_RS03665	789297..789956	+	660	WP_124967326.1	hemolysin III family protein	-
EH164_RS03670	790007..790987	-	981	WP_124967327.1	tRNA-modifying protein YgfZ	-
EH164_RS03675	791235..791501	+	267	WP_023480982.1	FAD assembly factor SdhE	Antitoxin
EH164_RS03680	791482..791898	+	417	WP_023480882.1	protein YgfX	Toxin
EH164_RS03685	791916..792437	-	522	WP_124967328.1	flavodoxin FldB	-
EH164_RS03690	792537..793433	+	897	WP_023480693.1	site-specific tyrosine recombinase XerD	-
EH164_RS03695	793458..794168	+	711	WP_124967329.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
EH164_RS03700	794174..795907	+	1734	WP_124967330.1	single-stranded-DNA-specific exonuclease RecJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-132)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 16438.36 Da
• Isoelectric Point: 10.7346

>T115538 WP_023480882.1 NZ_CP034225:791482-791898 [Kosakonia sp. CCTCC M2018092]
VVLWQSELRVSWRAQWLSLLLHGFVAALILLMPWPLSYTPVWLLLLSLVVFDSVRSQRRINACHGEIKLLMDSRLRWQGE
EWDIVGTPWMLRSGMMLRLRREEDGRRQHLWLAADSMDAQEWRDLRRMILQKPAQGLH

Nucleotide

• Length: 417 bp

>T115538 NZ_CP034225:791482-791898 [Kosakonia sp. CCTCC M2018092]
GTGGTCCTGTGGCAATCTGAGTTACGCGTCTCCTGGCGCGCACAGTGGCTCTCCTTACTGCTCCACGGATTCGTAGCCGC
CCTCATCTTGCTGATGCCCTGGCCGCTGAGCTACACGCCTGTATGGCTGCTGCTGCTTTCGCTGGTGGTGTTTGACTCCG
TACGGAGCCAACGGCGCATCAACGCCTGTCATGGCGAGATTAAGCTGCTGATGGATTCGCGCCTGCGCTGGCAGGGTGAG
GAGTGGGATATTGTCGGTACGCCCTGGATGCTGCGTAGCGGAATGATGCTGCGATTGCGTCGCGAGGAAGATGGGCGTCG
CCAGCATCTCTGGCTGGCGGCAGACAGTATGGATGCGCAGGAGTGGCGCGATCTGCGCCGGATGATTTTACAAAAACCGG
CGCAGGGGCTGCACTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10556.01 Da
• Isoelectric Point: 5.6186

>AT115538 WP_023480982.1 NZ_CP034225:791235-791501 [Kosakonia sp. CCTCC M2018092]
MDITNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDDKRLFVRLLENDDPDLFNWLMNHGKPQDAELQRMVQLIQTRN
RDRGPVAI

Nucleotide

• Length: 267 bp

>AT115538 NZ_CP034225:791235-791501 [Kosakonia sp. CCTCC M2018092]
ATGGATATTACTAACAAGGCCCGTATTCACTGGGCTTGCCGTCGCGGAATGCGCGAACTCGATATTTCCATCATGCCTTT
TTTCGAACATGAGTACGATTCGCTAAGCGATGATGACAAACGTCTCTTTGTCCGCCTGCTAGAAAATGACGATCCCGATT
TATTTAACTGGCTTATGAACCACGGCAAGCCACAGGATGCGGAGTTGCAAAGAATGGTGCAGTTAATCCAGACACGGAAT
CGGGATCGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A807LAC0