Detailed information of TA system TA115537

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-YafN
• Location: 626001..626520
• Replicon: chromosome
• Accession: NZ_CP034225
• Organism: Kosakonia sp. CCTCC M2018092

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: A0A7H0MI25
• Locus tag: EH164_RS03040
• Protein ID: WP_042717987.1
• Coordinates: 626001..626282 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: -
• Locus tag: EH164_RS03045
• Protein ID: WP_042717989.1
• Coordinates: 626272..626520 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EH164_RS03015	621320..621952	+	633	WP_124967242.1	ADP-ribose diphosphatase	-
EH164_RS03020	621949..622371	+	423	WP_023480711.1	DUF1249 family protein	-
EH164_RS03025	622396..623223	+	828	WP_124967243.1	3',5'-cyclic-AMP phosphodiesterase	-
EH164_RS03030	623223..623798	+	576	WP_023480978.1	esterase YqiA	-
EH164_RS03035	623838..625730	+	1893	WP_023481021.1	DNA topoisomerase IV subunit B	-
EH164_RS03040	626001..626282	-	282	WP_042717987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EH164_RS03045	626272..626520	-	249	WP_042717989.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
EH164_RS03050	626571..627446	-	876	WP_124967244.1	AraC family transcriptional regulator	-
EH164_RS03055	627612..629438	+	1827	WP_124967245.1	DUF2264 domain-containing protein	-
EH164_RS03060	629496..630755	+	1260	WP_023480794.1	oligosaccharide MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (7-84)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10914.71 Da
• Isoelectric Point: 10.6500

>T115537 WP_042717987.1 NZ_CP034225:c626282-626001 [Kosakonia sp. CCTCC M2018092]
MTYKLAFNVSALKEWKKLGSTVREQFKKKLQERLENPRVPAAQLHGRKDQYKIKLRGAGYRLVYKVEDEIITVTVIGVGK
RENDEIYTATRFR

Nucleotide

• Length: 282 bp

>T115537 NZ_CP034225:c626282-626001 [Kosakonia sp. CCTCC M2018092]
ATGACCTATAAGCTGGCGTTCAACGTATCGGCATTAAAGGAGTGGAAGAAGCTGGGAAGCACCGTTCGCGAGCAGTTCAA
AAAGAAGCTTCAGGAGCGCCTCGAAAACCCACGCGTCCCTGCAGCACAGCTCCACGGGCGTAAGGATCAGTACAAAATTA
AGCTACGCGGCGCGGGATACCGGCTGGTCTATAAGGTCGAGGATGAGATCATCACCGTGACGGTGATCGGTGTGGGCAAA
CGCGAGAACGATGAGATCTATACAGCCACACGCTTCAGGTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9097.37 Da
• Isoelectric Point: 3.9490

>AT115537 WP_042717989.1 NZ_CP034225:c626520-626272 [Kosakonia sp. CCTCC M2018092]
MPFQILTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYARLMEMLEDEELGRIADERADEPTIEVKLD
DL

Nucleotide

• Length: 249 bp

>AT115537 NZ_CP034225:c626520-626272 [Kosakonia sp. CCTCC M2018092]
ATGCCATTCCAGATTCTGACGACCACCGCAGCCAGCATCACCGAACTGAAACGTGACCCGATGGGGACATTTAATGCCGG
GGAAGGCGCGCCGGTTGCCATTCTTAATCGCAATGAACCCGCGTTCTACTGCGTTCCGCCCGCGCTCTATGCACGCCTGA
TGGAGATGCTCGAAGACGAAGAGCTGGGGCGCATTGCGGACGAGCGTGCTGATGAACCCACCATCGAGGTCAAGCTGGAT
GACCTATAA

Structures

Toxin

• AlphaFold DB: A0A7H0MI25