Detailed information of TA system TA115517

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 722611..723206
• Replicon: chromosome
• Accession: NZ_CP034213
• Organism: Escherichia albertii strain NCTC 9362

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: DSB70_RS03750
• Protein ID: WP_125059429.1
• Coordinates: 722829..723206 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: B1ELZ9
• Locus tag: DSB70_RS03745
• Protein ID: WP_001195490.1
• Coordinates: 722611..722832 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DSB70_RS03725 (717952)	717952..719112	+	1161	WP_125060733.1	diguanylate cyclase	-
DSB70_RS03730 (719359)	719359..720810	+	1452	WP_044716705.1	tagaturonate reductase	-
DSB70_RS03735 (721015)	721015..721929	+	915	WP_024164792.1	bestrophin family protein	-
DSB70_RS03740 (721933)	721933..722355	-	423	Protein_737	trans-aconitate 2-methyltransferase	-
DSB70_RS03745 (722611)	722611..722832	+	222	WP_001195490.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
DSB70_RS03750 (722829)	722829..723206	+	378	WP_125059429.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
DSB70_RS24485 (723209)	723209..723679	-	471	WP_125059431.1	autotransporter outer membrane beta-barrel domain-containing protein	-
DSB70_RS03760 (723676)	723676..724998	-	1323	WP_125059432.1	autotransporter outer membrane beta-barrel domain-containing protein	-
DSB70_RS03765 (724896)	724896..725963	-	1068	WP_125059434.1	ESPR-type extended signal peptide-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (3-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-53)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14134.08 Da
• Isoelectric Point: 7.4005

>T115517 WP_125059429.1 NZ_CP034213:722829-723206 [Escherichia albertii]
MIWVSAQEVIAFHDRILQRFPGVAGLTDPGRAQALIYRVQNRVHYEGVTDLFELAATYWVAIARGHIFHDGNKRTAFFVT
MTFLWRNGIRIRDVDNSLENLTVEAATGEKTVGQFTYAPTLSASF

Nucleotide

• Length: 378 bp

>T115517 NZ_CP034213:722829-723206 [Escherichia albertii]
ATGATTTGGGTCAGTGCACAGGAGGTTATTGCCTTCCACGATCGAATTTTGCAACGTTTTCCCGGTGTGGCGGGCCTGAC
TGACCCCGGCAGAGCACAGGCGTTGATTTATCGCGTGCAGAATCGTGTTCACTACGAGGGCGTTACTGACCTGTTTGAAC
TGGCAGCAACCTACTGGGTTGCCATCGCTCGCGGTCACATCTTTCATGATGGTAATAAACGTACCGCATTTTTCGTTACT
ATGACCTTTCTTTGGCGCAATGGCATCCGTATCCGTGACGTTGATAACTCGCTGGAGAACCTGACTGTCGAAGCGGCTAC
CGGAGAAAAAACGGTCGGTCAATTTACATATGCCCCTACTTTGTCGGCGTCGTTCTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8067.96 Da
• Isoelectric Point: 4.6816

>AT115517 WP_001195490.1 NZ_CP034213:722611-722832 [Escherichia albertii]
MQTYTSTQARANIATVLDTALNGEPVEITRRDGSSAVLISKAAFEAYQNAKLDAEFDAIMQRHEHTIEALTDR

Nucleotide

• Length: 222 bp

>AT115517 NZ_CP034213:722611-722832 [Escherichia albertii]
ATGCAAACTTACACTTCCACACAAGCCAGAGCCAATATTGCCACTGTACTGGATACCGCGTTGAATGGTGAGCCAGTTGA
AATCACCCGTCGCGACGGCAGCTCTGCCGTGCTCATTAGTAAAGCCGCATTCGAGGCGTATCAAAATGCGAAACTGGACG
CGGAATTTGATGCCATCATGCAACGTCATGAACACACAATTGAAGCGTTGACTGATCGATGA

Structures

Antitoxin

• AlphaFold DB: A0A2S6PD20