Detailed information of TA system TA115474

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1240352..1241269
• Replicon: chromosome
• Accession: NZ_CP034203
• Organism: Bacillus velezensis strain 83

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: EBA29_RS06415
• Protein ID: WP_094295697.1
• Coordinates: 1240523..1241269 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: EBA29_RS06410
• Protein ID: WP_003154807.1
• Coordinates: 1240352..1240522 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EBA29_RS06375	1235585..1237207	+	1623	WP_072177386.1	pyocin knob domain-containing protein	-
EBA29_RS06380	1237220..1237591	+	372	WP_017417608.1	hypothetical protein	-
EBA29_RS06385	1237596..1237793	+	198	WP_007610833.1	XkdX family protein	-
EBA29_RS06390	1237850..1238611	+	762	WP_094295696.1	phage portal protein	-
EBA29_RS06395	1238663..1238926	+	264	WP_022553473.1	hemolysin XhlA family protein	-
EBA29_RS06400	1238940..1239203	+	264	WP_003154813.1	phage holin	-
EBA29_RS06405	1239217..1240095	+	879	WP_007407257.1	N-acetylmuramoyl-L-alanine amidase	-
EBA29_RS06410	1240352..1240522	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
EBA29_RS06415	1240523..1241269	-	747	WP_094295697.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
EBA29_RS06420	1241374..1242372	-	999	WP_003154805.1	inorganic phosphate transporter	-
EBA29_RS06425	1242385..1243002	-	618	WP_003154804.1	DUF47 domain-containing protein	-
EBA29_RS06430	1243288..1244604	-	1317	WP_007610842.1	amino acid permease	-
EBA29_RS06435	1244926..1245876	+	951	WP_022553472.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29008.46 Da
• Isoelectric Point: 4.6826

>T115474 WP_094295697.1 NZ_CP034203:c1241269-1240523 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWSQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T115474 NZ_CP034203:c1241269-1240523 [Bacillus velezensis]
ATGCTTTTGTTTTTTCAGATTATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTACTGGGCTCCATGGTCTATTGGA
CATATGATCCTGAATCGCTCTTTGCCGCATGGAGTCAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCCGCTTATGCCGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCAGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAGAACCGCCATTATGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT115474 WP_003154807.1 NZ_CP034203:c1240522-1240352 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT115474 NZ_CP034203:c1240522-1240352 [Bacillus velezensis]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Structures

Antitoxin

• AlphaFold DB: I2HQ14