Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 468665..469302 Replicon chromosome
Accession NZ_CP034203
Organism Bacillus velezensis strain 83

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag EBA29_RS02320 Protein ID WP_003156187.1
Coordinates 468952..469302 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag EBA29_RS02315 Protein ID WP_003156188.1
Coordinates 468665..468946 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EBA29_RS02295 465030..465629 - 600 WP_128496462.1 rhomboid family intramembrane serine protease -
EBA29_RS02300 465722..466087 + 366 WP_014304402.1 holo-ACP synthase -
EBA29_RS02305 466252..467259 + 1008 WP_007410230.1 outer membrane lipoprotein carrier protein LolA -
EBA29_RS02310 467376..468545 + 1170 WP_021495079.1 alanine racemase -
EBA29_RS02315 468665..468946 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
EBA29_RS02320 468952..469302 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
EBA29_RS02325 469420..470241 + 822 WP_003156182.1 STAS domain-containing protein -
EBA29_RS02330 470246..470611 + 366 WP_007609584.1 RsbT antagonist protein RsbS -
EBA29_RS02335 470614..471015 + 402 WP_003156178.1 anti-sigma regulatory factor -
EBA29_RS02340 471027..472034 + 1008 WP_007609589.1 PP2C family protein-serine/threonine phosphatase -
EBA29_RS02345 472098..472427 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
EBA29_RS02350 472424..472906 + 483 WP_007609591.1 anti-sigma B factor RsbW -
EBA29_RS02355 472872..473660 + 789 WP_029974100.1 RNA polymerase sigma factor SigB -
EBA29_RS02360 473660..474262 + 603 WP_128496463.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T115473 WP_003156187.1 NZ_CP034203:468952-469302 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T115473 NZ_CP034203:468952-469302 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT115473 WP_003156188.1 NZ_CP034203:468665-468946 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT115473 NZ_CP034203:468665-468946 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References