Detailed information of TA system TA115471

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 1536720..1537324
• Replicon: chromosome
• Accession: NZ_CP034192
• Organism: Bifidobacterium breve strain lw01

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: EH245_RS06935
• Protein ID: WP_080988839.1
• Coordinates: 1536720..1537037 (+)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: EH245_RS06940
• Protein ID: WP_052789767.1
• Coordinates: 1537031..1537324 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EH245_RS06920	1532693..1533670	-	978	WP_052789765.1	carbohydrate ABC transporter permease	-
EH245_RS06925	1533670..1534581	-	912	WP_052789766.1	sugar ABC transporter permease	-
EH245_RS06930	1534939..1535981	+	1043	Protein_1299	LacI family DNA-binding transcriptional regulator	-
EH245_RS06935	1536720..1537037	+	318	WP_080988839.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EH245_RS06940	1537031..1537324	+	294	WP_052789767.1	putative addiction module antidote protein	Antitoxin
EH245_RS10520	1537375..1537650	-	276	WP_155465850.1	hypothetical protein	-
EH245_RS06955	1537872..1538508	+	637	Protein_1303	hypothetical protein	-
EH245_RS06960	1538579..1539061	-	483	Protein_1304	hypothetical protein	-
EH245_RS06965	1539143..1541062	-	1920	WP_065453776.1	ABC transporter ATP-binding protein	-
EH245_RS06970	1541066..1541803	-	738	WP_201786398.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (4-98)

Antitoxin

• Uncharacterized protein HI1420 (4-89)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 11943.85 Da
• Isoelectric Point: 10.5847

>T115471 WP_080988839.1 NZ_CP034192:1536720-1537037 [Bifidobacterium breve]
VHVEIQQTDQFRKWLHRLRDHAAKARIAARIRQCALAGQPVGDMHPVGKGVNELRFFFGPGYRVYFAQKGNQIMLLLAGG
DKTGQDKTIKEAQKMLESLIKEGQW

Nucleotide

• Length: 318 bp

>T115471 NZ_CP034192:1536720-1537037 [Bifidobacterium breve]
GTGCATGTGGAAATCCAACAAACCGACCAGTTCCGCAAGTGGTTGCACAGACTGCGCGACCATGCCGCCAAAGCGCGGAT
CGCTGCCCGGATCAGACAGTGTGCACTGGCGGGACAACCGGTCGGCGATATGCACCCGGTCGGTAAAGGCGTGAACGAAT
TGCGTTTCTTCTTCGGCCCAGGCTACCGCGTCTACTTCGCCCAGAAAGGCAATCAAATCATGTTGCTGCTCGCCGGCGGG
GACAAGACCGGACAGGACAAGACCATAAAAGAGGCACAGAAAATGCTCGAATCACTTATCAAGGAGGGACAATGGTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10191.52 Da
• Isoelectric Point: 4.3094

>AT115471 WP_052789767.1 NZ_CP034192:1537031-1537324 [Bifidobacterium breve]
MVNISNFDASEYIENEEDAIAYLNAAAETGDPALLQAAIGDIAKARGMGQIAKEAGVGRESLYKSLNKEGNPSFRTIVKV
VDALGGRLVIEPLPATA

Nucleotide

• Length: 294 bp

>AT115471 NZ_CP034192:1537031-1537324 [Bifidobacterium breve]
ATGGTAAACATCAGCAATTTTGATGCCAGCGAATACATCGAAAACGAAGAAGACGCCATCGCATACCTCAACGCCGCCGC
GGAAACCGGCGACCCGGCGCTTCTGCAGGCCGCCATCGGCGACATAGCCAAGGCGCGTGGCATGGGCCAAATCGCCAAGG
AAGCCGGAGTCGGCCGCGAAAGCCTCTACAAAAGCCTCAACAAAGAAGGCAACCCAAGTTTCCGCACCATCGTCAAGGTC
GTCGATGCACTTGGAGGCCGACTTGTCATCGAACCCTTGCCCGCCACGGCATGA