Detailed information of TA system TA115470

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YafQ-RelB
• Location: 1261535..1262101
• Replicon: chromosome
• Accession: NZ_CP034192
• Organism: Bifidobacterium breve strain lw01

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: EH245_RS05750
• Protein ID: WP_124880060.1
• Coordinates: 1261808..1262101 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: EH245_RS05745
• Protein ID: WP_124880059.1
• Coordinates: 1261535..1261804 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EH245_RS10485	1257055..1257273	-	219	WP_050824881.1	hypothetical protein	-
EH245_RS05710	1257442..1258455	-	1014	WP_164463530.1	VanZ family protein	-
EH245_RS05715	1258602..1259208	+	607	Protein_1064	aminotransferase class V-fold PLP-dependent enzyme	-
EH245_RS05720	1259262..1259426	+	165	Protein_1065	aminotransferase class V	-
EH245_RS05725	1259495..1259794	+	300	WP_016462931.1	DUF2316 family protein	-
EH245_RS05730	1259835..1260368	-	534	WP_003829402.1	GNAT family N-acetyltransferase	-
EH245_RS05735	1260725..1260844	+	120	WP_164463531.1	IS3 family transposase	-
EH245_RS05740	1260853..1261350	+	498	Protein_1069	FtsX-like permease family protein	-
EH245_RS05745	1261535..1261804	+	270	WP_124880059.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
EH245_RS05750	1261808..1262101	+	294	WP_124880060.1	type II toxin-antitoxin system YafQ family toxin	Toxin
EH245_RS05760	1262770..1263321	+	552	WP_052789591.1	RNA polymerase sigma factor	-
EH245_RS05765	1263355..1264182	+	828	WP_124880062.1	hypothetical protein	-
EH245_RS05770	1264313..1264939	+	627	WP_124880063.1	hypothetical protein	-
EH245_RS05775	1264936..1265718	+	783	WP_124880064.1	murein hydrolase transporter LrgB	-
EH245_RS05780	1265771..1266646	+	876	WP_124880065.1	ABC transporter ATP-binding protein	-
EH245_RS05785	1266700..1267098	+	399	WP_124880066.1	superoxide dismutase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	tet(W)	-	1259495..1281167	21672

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (3-95)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (3-88)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11262.95 Da
• Isoelectric Point: 8.1116

>T115470 WP_124880060.1 NZ_CP034192:1261808-1262101 [Bifidobacterium breve]
MLKPDYTAAFQRDIKKLKRKHADLRPLKEVIRLVLEDTADSKEALRRRHRAHTLTGDLNDVLECHIGNDGDWLLLWIRDD
GTAMFMRTGSHDELLGK

Nucleotide

• Length: 294 bp

>T115470 NZ_CP034192:1261808-1262101 [Bifidobacterium breve]
ATGCTCAAACCGGATTACACGGCGGCATTCCAGCGAGATATCAAGAAGCTCAAACGCAAGCACGCTGATTTACGCCCGCT
GAAAGAAGTCATTCGTCTGGTGCTTGAGGACACGGCGGACTCCAAGGAAGCATTGAGGCGTAGGCATCGCGCCCACACAC
TTACCGGTGATTTAAACGACGTACTGGAATGCCACATCGGCAATGACGGCGACTGGCTGCTTCTATGGATTCGCGATGAT
GGTACCGCCATGTTCATGCGAACCGGCAGCCATGACGAACTGTTGGGCAAATAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9833.14 Da
• Isoelectric Point: 4.6050

>AT115470 WP_124880059.1 NZ_CP034192:1261535-1261804 [Bifidobacterium breve]
VSMASVTVRVDADTKQKAADIAEDLGLDLSSVTRAFYKQIVREKRIPLNLSYTTVPPETMEALDEAKNRLADGKPRFNSA
DEMFDSLGI

Nucleotide

• Length: 270 bp

>AT115470 NZ_CP034192:1261535-1261804 [Bifidobacterium breve]
GTGAGCATGGCATCTGTCACAGTCCGCGTGGATGCGGACACCAAGCAGAAAGCCGCAGACATAGCCGAGGATCTCGGTCT
GGACTTGTCTTCGGTCACCCGCGCCTTCTACAAGCAGATTGTCCGGGAGAAGCGCATTCCCCTAAACCTTTCCTATACGA
CCGTGCCGCCTGAAACCATGGAGGCCCTTGACGAGGCGAAAAACAGGCTCGCCGACGGCAAACCTCGCTTCAACAGTGCC
GATGAGATGTTCGATTCGTTGGGAATCTGA