Detailed information of TA system TA115469

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/RelB(antitoxin)
• Location: 1086142..1086778
• Replicon: chromosome
• Accession: NZ_CP034192
• Organism: Bifidobacterium breve strain lw01

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: EH245_RS04885
• Protein ID: WP_124880005.1
• Coordinates: 1086142..1086498 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: D6PAX1
• Locus tag: EH245_RS04890
• Protein ID: WP_012577921.1
• Coordinates: 1086485..1086778 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EH245_RS04855	1081196..1082107	+	912	WP_025221542.1	ABC transporter ATP-binding protein	-
EH245_RS04860	1082304..1082930	+	627	WP_003829226.1	30S ribosomal protein S4	-
EH245_RS04865	1083405..1084187	-	783	WP_124880004.1	gamma-glutamyl-gamma-aminobutyrate hydrolase family protein	-
EH245_RS04870	1084325..1084810	-	486	WP_025221544.1	cytidine deaminase	-
EH245_RS04875	1084901..1085257	+	357	WP_025221545.1	SdpI family protein	-
EH245_RS04880	1085362..1085964	+	603	WP_003829233.1	transglutaminase family protein	-
EH245_RS04885	1086142..1086498	-	357	WP_124880005.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
EH245_RS04890	1086485..1086778	-	294	WP_012577921.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
EH245_RS04895	1087030..1087704	+	675	WP_124880006.1	histidine phosphatase family protein	-
EH245_RS04900	1087787..1088170	+	384	WP_016463075.1	DUF948 domain-containing protein	-
EH245_RS04905	1088170..1088406	+	237	WP_003829239.1	hypothetical protein	-
EH245_RS04910	1088502..1091180	+	2679	WP_065454086.1	alanine--tRNA ligase	-
EH245_RS04915	1091189..1091641	+	453	WP_003829241.1	Holliday junction resolvase RuvX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-111)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (11-53)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13283.20 Da
• Isoelectric Point: 7.9984

>T115469 WP_124880005.1 NZ_CP034192:c1086498-1086142 [Bifidobacterium breve]
MMKTDPRQFEIWWVPFAFPDKPGKAKNRPSVILQWDDGTHIALVTKVTGNTWRDEPGYVVLRDWQDAGLSKPSAVRCSQL
LRLPSNLFLDDGPTGRLSAYDAGRVAWAINELYPGLLA

Nucleotide

• Length: 357 bp

>T115469 NZ_CP034192:c1086498-1086142 [Bifidobacterium breve]
ATGATGAAGACTGATCCGCGCCAGTTCGAGATATGGTGGGTGCCGTTCGCTTTTCCCGACAAGCCAGGCAAAGCGAAGAA
CCGTCCCAGCGTGATCCTCCAATGGGACGACGGCACCCACATCGCCTTGGTGACCAAGGTCACCGGCAACACCTGGCGCG
ACGAACCGGGCTATGTGGTGCTGCGTGACTGGCAGGACGCGGGACTGAGCAAGCCCAGCGCCGTGCGATGCTCGCAACTG
CTGCGGCTGCCAAGCAATCTTTTCCTCGACGACGGCCCGACAGGAAGACTTTCGGCATATGATGCCGGCCGCGTCGCCTG
GGCGATCAACGAACTCTATCCGGGCCTACTAGCCTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10866.31 Da
• Isoelectric Point: 4.2896

>AT115469 WP_012577921.1 NZ_CP034192:c1086778-1086485 [Bifidobacterium breve]
MAIKEKTTISLDAQTKRDGIAILDAMGLNLSTFAEMSLRQLVRDGRLPFTPSVRPSFEKDNEGYPLFKANMDDPRIVTPQ
IRDGAVILPEGWDDDED

Nucleotide

• Length: 294 bp

>AT115469 NZ_CP034192:c1086778-1086485 [Bifidobacterium breve]
ATGGCTATCAAGGAGAAGACCACGATCAGTCTGGATGCGCAGACCAAGCGTGACGGCATCGCCATACTTGACGCCATGGG
GTTGAACCTGAGCACGTTTGCCGAAATGTCTCTCAGACAATTGGTACGCGACGGGCGTCTGCCATTCACGCCAAGCGTCC
GCCCTTCATTCGAAAAAGACAACGAGGGATATCCCCTGTTCAAAGCGAACATGGATGATCCCCGCATCGTGACGCCCCAG
ATACGTGATGGTGCCGTGATCCTGCCGGAGGGTTGGGACGATGATGAAGACTGA

Structures

Antitoxin

• AlphaFold DB: A0A1S2VTZ6