Detailed information of TA system TA115464

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 2648136..2649735
• Replicon: chromosome
• Accession: NZ_CP034182
• Organism: Bordetella pertussis strain BPD1

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: Q7VVM6
• Locus tag: EHO76_RS12835
• Protein ID: WP_003816876.1
• Coordinates: 2648136..2649476 (-)
• Length: 447 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: EHO76_RS12840
• Protein ID: WP_019247974.1
• Coordinates: 2649478..2649735 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EHO76_RS12815	2643362..2644183	-	822	WP_014905903.1	IclR family transcriptional regulator	-
EHO76_RS12820	2644598..2645676	-	1079	Protein_2486	tellurite resistance/C4-dicarboxylate transporter family protein	-
EHO76_RS12825	2645908..2647338	-	1431	WP_019247976.1	MFS transporter	-
EHO76_RS12830	2647351..2648040	+	690	WP_010931038.1	TetR/AcrR family transcriptional regulator	-
EHO76_RS12835	2648136..2649476	-	1341	WP_003816876.1	type II toxin-antitoxin system HipA family toxin	Toxin
EHO76_RS12840	2649478..2649735	-	258	WP_019247974.1	helix-turn-helix domain-containing protein	Antitoxin
EHO76_RS12850	2650089..2651681	-	1593	WP_010931040.1	glutamine-hydrolyzing GMP synthase	-
EHO76_RS12855	2651778..2653238	-	1461	WP_003812368.1	IMP dehydrogenase	-
EHO76_RS12860	2653461..2654072	+	612	WP_010931041.1	DUF2239 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (10-112)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-70)

Sequences

Toxin

Protein

• Length: 447 a.a.
• Molecular weight: 49632.79 Da
• Isoelectric Point: 7.4158

>T115464 WP_003816876.1 NZ_CP034182:c2649476-2648136 [Bordetella pertussis]
MGRRSHSRSLSIWTNGVRVGRWTVPARGEMELQYDVDWVRADVGRPLSLSLPFNLQNLPLKGERVANYFDNLLPDSDAIR
RRVAERFRTGSVDPFDLLKAIGRDCVGAVQILEDEEIPEGFDRIEGVPLSEEDIERHLIETVTPRAFAAGRDPDADFRIS
LAGAQEKTAFLWWGGQWLAPRGATSTSHIFKLPLGLVGGRRADFSTSVDNEWLCLKLLKAYGLDVAEASIATFGQQRVLV
VERFDRRVAPNGQWLMRLPQEDFCQVEGCSPLRKYENEGGPGLKALFATLRQSVNAAVDMRTLMAAQILFWLLRAPDGHA
KNFSIQLLPRGRFQLTRLYDVMSAYPVLGDGANQWSPFEIKLAMALIGKNRHYEMHGIQRRHFNSTAQKVGYGPTAEPII
EDLLARTPAIIAAVQADLPQDFSPRVADSVLGGLAQAAKSLGAMMP

Nucleotide

• Length: 1341 bp

>T115464 NZ_CP034182:c2649476-2648136 [Bordetella pertussis]
ATGGGGCGCCGATCGCACAGCCGCAGCCTGTCCATCTGGACCAACGGCGTTCGCGTCGGGCGTTGGACGGTTCCCGCTCG
CGGAGAGATGGAACTGCAGTACGACGTGGATTGGGTCCGTGCCGACGTCGGCCGGCCACTTTCGCTGTCGCTGCCATTCA
ACTTGCAGAATCTGCCCCTCAAGGGGGAGAGAGTCGCCAACTACTTCGACAATCTGCTACCGGACAGCGACGCTATCCGT
CGTCGCGTGGCCGAGCGATTCAGGACGGGCTCGGTCGATCCCTTCGATCTGTTGAAAGCCATTGGACGCGATTGCGTTGG
CGCCGTACAGATCCTGGAAGACGAAGAGATACCTGAGGGCTTTGATCGCATCGAGGGCGTGCCTCTGTCCGAGGAGGACA
TCGAGCGGCATTTGATCGAAACCGTTACGCCGCGCGCCTTCGCTGCGGGCCGGGACCCGGACGCGGATTTCAGAATTTCT
CTGGCCGGCGCGCAAGAGAAGACAGCCTTCCTGTGGTGGGGCGGACAGTGGCTTGCGCCGCGTGGCGCGACATCGACGAG
CCATATTTTCAAACTTCCATTGGGCTTGGTGGGTGGACGGCGGGCTGACTTCAGCACTTCTGTTGATAACGAATGGCTGT
GTCTGAAGCTGCTGAAGGCGTATGGGTTGGATGTGGCTGAGGCCAGCATCGCAACCTTCGGACAGCAGCGAGTGCTGGTG
GTCGAGCGATTCGACCGGCGTGTTGCGCCCAACGGGCAATGGCTGATGCGCCTGCCGCAGGAAGATTTCTGCCAGGTCGA
GGGGTGCTCGCCGCTACGCAAGTATGAAAACGAGGGCGGCCCAGGACTCAAGGCCTTATTCGCCACGCTGCGGCAGTCAG
TGAACGCAGCGGTCGATATGAGAACGTTGATGGCGGCGCAGATCCTGTTTTGGTTGCTGCGGGCACCCGATGGCCACGCC
AAGAATTTCAGCATCCAGTTGTTGCCGCGCGGCCGTTTCCAGTTGACTAGACTGTACGACGTGATGTCGGCCTATCCGGT
ACTGGGCGATGGCGCGAATCAGTGGTCGCCGTTCGAAATCAAATTGGCGATGGCATTGATTGGCAAGAACCGGCATTACG
AGATGCACGGCATCCAGCGCAGGCACTTCAACAGCACCGCACAAAAAGTTGGTTATGGCCCGACGGCGGAGCCGATCATT
GAAGACCTGTTGGCCCGTACGCCCGCGATCATTGCCGCCGTGCAGGCCGATTTGCCGCAGGACTTCTCGCCGCGTGTTGC
GGACTCCGTCCTGGGTGGGCTTGCGCAGGCCGCAAAGTCGCTCGGCGCAATGATGCCGTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 8815.04 Da
• Isoelectric Point: 7.1642

>AT115464 WP_019247974.1 NZ_CP034182:c2649735-2649478 [Bordetella pertussis]
MPPPSQPLLTTGQLGAVLQAARKAQGLTQSALASRIGLSQSRVSHLELNAHQLSVEQLLAWCAALGLELTIATRGSPAGS
SDADW

Nucleotide

• Length: 258 bp

>AT115464 NZ_CP034182:c2649735-2649478 [Bordetella pertussis]
ATGCCGCCGCCATCACAACCTCTGCTCACTACCGGCCAGCTTGGCGCGGTCTTGCAGGCTGCACGCAAAGCACAGGGACT
GACTCAATCGGCCCTGGCCAGTCGTATCGGCCTGAGCCAATCGCGGGTCTCGCACCTGGAGTTGAATGCCCATCAACTGA
GCGTGGAGCAGCTCCTGGCCTGGTGCGCGGCGCTAGGGTTGGAGTTGACGATTGCGACTCGCGGAAGTCCCGCAGGGTCT
TCCGACGCGGATTGGTGA

Structures

Toxin

• AlphaFold DB: Q7VVM6