Detailed information of TA system TA115460

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3602866..3603668
• Replicon: chromosome
• Accession: NZ_CP034179
• Organism: Novosphingobium tardaugens NBRC 16725

Toxin (Protein)

• Gene name: TacT3
• Uniprot ID: A0A126RRI7
• Locus tag: EGO55_RS16940
• Protein ID: WP_030090520.1
• Coordinates: 3603141..3603668 (+)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: TacA3
• Uniprot ID: -
• Locus tag: EGO55_RS16935
• Protein ID: WP_030090519.1
• Coordinates: 3602866..3603144 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGO55_RS16935	3602866..3603144	+	279	WP_030090519.1	DUF1778 domain-containing protein	Antitoxin
EGO55_RS16940	3603141..3603668	+	528	WP_030090520.1	hypothetical protein	Toxin
EGO55_RS16945	3604004..3604345	+	342	WP_021688185.1	sigma-70 family RNA polymerase sigma factor	-
EGO55_RS16950	3604773..3605993	+	1221	WP_021688184.1	hypothetical protein	-
EGO55_RS16955	3606217..3606864	+	648	WP_021688183.1	hypothetical protein	-
EGO55_RS16960	3606876..3607337	+	462	WP_021688182.1	hypothetical protein	-
EGO55_RS16965	3607381..3608136	+	756	WP_021688181.1	hypothetical protein	-
EGO55_RS16970	3608142..3608621	+	480	WP_021688180.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	3586960..3773867	186907

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (11-88)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 19149.84 Da
• Isoelectric Point: 7.9468

>T115460 WP_030090520.1 NZ_CP034179:3603141-3603668 [Novosphingobium tardaugens NBRC 16725]
MRLPSWHEDAIAKAHDRASFDCGDNDLNIFLRRYARQSHELGGAKTFLAVDDTDNKTILGFYSIAPGSMDHADTPELARR
GLARHEVPGFRLARLATDIRVQGNGLGGQLLGAIGRRCIRVAAEVGGVMLIIDAKNERAAAWYASYGAITLHDKPLTLIL
PFATLERELRSAGQL

Nucleotide

• Length: 528 bp

>T115460 NZ_CP034179:3603141-3603668 [Novosphingobium tardaugens NBRC 16725]
ATGAGACTTCCGTCCTGGCACGAGGACGCGATCGCAAAAGCGCATGACCGAGCTTCCTTCGACTGCGGTGACAATGATCT
GAACATCTTCTTGCGGCGCTATGCCCGGCAAAGCCATGAGCTGGGCGGGGCCAAGACGTTCCTTGCCGTCGACGACACCG
ACAACAAGACGATTCTCGGTTTCTACAGCATCGCTCCTGGCAGCATGGACCATGCCGACACGCCCGAACTTGCGAGGCGG
GGCCTCGCCAGGCATGAAGTGCCCGGTTTCCGGCTCGCGCGGCTTGCAACCGATATTCGGGTGCAGGGGAACGGGCTTGG
GGGGCAACTTCTTGGCGCCATCGGCCGCAGATGCATTCGCGTCGCGGCAGAAGTCGGCGGGGTGATGCTGATCATCGACG
CCAAGAACGAGCGGGCTGCCGCATGGTATGCCAGCTATGGCGCCATAACACTGCACGACAAACCGCTGACCCTCATATTG
CCTTTTGCGACACTTGAACGTGAATTGAGATCAGCGGGTCAATTGTAG

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10156.81 Da
• Isoelectric Point: 10.2844

>AT115460 WP_030090519.1 NZ_CP034179:3602866-3603144 [Novosphingobium tardaugens NBRC 16725]
MPRAVVEDNRRMSLRIRPEQKALLARAAALRHTDLTTFVTGSALREAESVIEEAERTKLSERDSLLVLDLLENPPPANAK
LRAAIAAMPKLA

Nucleotide

• Length: 279 bp

>AT115460 NZ_CP034179:3602866-3603144 [Novosphingobium tardaugens NBRC 16725]
ATGCCGCGAGCTGTGGTGGAAGACAATAGGCGCATGAGCCTGCGCATACGACCGGAGCAAAAGGCTTTGCTCGCCCGGGC
GGCGGCGCTCAGACATACCGATCTGACGACCTTTGTCACCGGTTCGGCGCTGCGCGAGGCCGAGAGCGTAATCGAAGAGG
CGGAGCGGACCAAGCTGAGCGAACGGGACAGCCTGCTCGTTCTCGATCTGCTCGAAAATCCCCCTCCGGCGAATGCAAAA
TTGCGCGCTGCCATCGCCGCGATGCCCAAGCTCGCATGA

Structures

Toxin

• AlphaFold DB: A0A126RRI7