Detailed information of TA system TA115458

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1703995..1704802
• Replicon: chromosome
• Accession: NZ_CP034179
• Organism: Novosphingobium tardaugens NBRC 16725

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: U2YBZ0
• Locus tag: EGO55_RS07745
• Protein ID: WP_021691879.1
• Coordinates: 1703995..1704498 (-)
• Length: 168 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: U2YQS6
• Locus tag: EGO55_RS07750
• Protein ID: WP_021691880.1
• Coordinates: 1704503..1704802 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGO55_RS07730	1701193..1702140	-	948	WP_021691876.1	hypothetical protein	-
EGO55_RS07735	1702140..1703090	-	951	WP_021691877.1	DUF1738 domain-containing protein	-
EGO55_RS07740	1703166..1703987	-	822	WP_021691878.1	hypothetical protein	-
EGO55_RS07745	1703995..1704498	-	504	WP_021691879.1	GNAT family N-acetyltransferase	Toxin
EGO55_RS07750	1704503..1704802	-	300	WP_021691880.1	DUF1778 domain-containing protein	Antitoxin
EGO55_RS07755	1705071..1705295	+	225	WP_021691881.1	hypothetical protein	-
EGO55_RS07760	1705315..1706967	+	1653	WP_124916855.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1646385..1706933	60548

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (43-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (3-92)

Sequences

Toxin

Protein

• Length: 168 a.a.
• Molecular weight: 17652.43 Da
• Isoelectric Point: 7.8301

>T115458 WP_021691879.1 NZ_CP034179:c1704498-1703995 [Novosphingobium tardaugens NBRC 16725]
MSLSAPEPITVVHDVSQFSCGKPTLDHWLKARALSNQQKGFTAVLVVHEAGRVVGYYGLAPTAVVPAVLPRAIRTGQPPD
PVPCLLLGQLATDEAWVGQGIGTGLVKHALQRCVQAAGLIGGRALMVNAVDDDAAQFWQRRGFMPTHDDPLVLFRSIAAI
AASLAQA

Nucleotide

• Length: 504 bp

>T115458 NZ_CP034179:c1704498-1703995 [Novosphingobium tardaugens NBRC 16725]
TTGTCCCTTTCGGCTCCTGAACCGATCACGGTCGTGCATGACGTTTCGCAATTCTCCTGCGGCAAGCCGACGCTCGATCA
CTGGCTGAAAGCCCGCGCCCTATCGAACCAGCAAAAGGGCTTTACCGCCGTTCTCGTGGTGCATGAGGCCGGGCGGGTTG
TCGGCTACTACGGTCTGGCGCCCACGGCCGTCGTGCCCGCTGTCCTGCCGCGTGCGATCCGCACCGGACAGCCGCCCGAT
CCGGTTCCGTGCCTGCTTCTCGGCCAACTCGCCACGGACGAAGCCTGGGTGGGGCAAGGGATCGGCACTGGTCTCGTGAA
ACATGCGCTCCAGCGCTGTGTGCAAGCTGCCGGCCTGATCGGCGGGCGGGCGTTGATGGTCAATGCCGTCGACGACGACG
CTGCGCAGTTCTGGCAGCGGCGCGGCTTTATGCCGACGCATGACGATCCGCTCGTTCTCTTCCGGTCCATCGCTGCGATC
GCCGCGTCGCTGGCGCAGGCATAG

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10963.70 Da
• Isoelectric Point: 5.2090

>AT115458 WP_021691880.1 NZ_CP034179:c1704802-1704503 [Novosphingobium tardaugens NBRC 16725]
MASTAERKEYPISMRLPEADVAMIDRAATLRGRSRTDFVRDAAVRAAEEVVMEQSLIRMSPEGFAGFMEILAAPALPVPA
MVELAKRPAPWESGYEPKR

Nucleotide

• Length: 300 bp

>AT115458 NZ_CP034179:c1704802-1704503 [Novosphingobium tardaugens NBRC 16725]
ATGGCCAGCACAGCAGAGCGCAAGGAATATCCCATCTCGATGCGATTGCCCGAGGCTGATGTCGCGATGATCGATCGAGC
CGCTACGCTGCGGGGTCGCTCGCGCACCGACTTCGTGCGCGACGCCGCCGTGCGCGCGGCCGAGGAAGTGGTTATGGAGC
AAAGCCTGATCCGCATGAGCCCGGAAGGGTTCGCCGGGTTTATGGAGATTCTGGCCGCCCCCGCACTGCCGGTTCCCGCG
ATGGTCGAGCTGGCCAAGCGCCCGGCGCCCTGGGAGTCCGGTTACGAGCCGAAGCGGTAA

Structures

Toxin

• AlphaFold DB: U2YBZ0

Antitoxin

• AlphaFold DB: U2YQS6