Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 486079..486716 Replicon chromosome
Accession NZ_CP034176
Organism Bacillus velezensis strain MH25

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag BVMH_RS02485 Protein ID WP_003156187.1
Coordinates 486366..486716 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag BVMH_RS02480 Protein ID WP_003156188.1
Coordinates 486079..486360 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BVMH_RS02460 482444..483043 - 600 WP_015239195.1 rhomboid family intramembrane serine protease -
BVMH_RS02465 483136..483501 + 366 WP_094031521.1 holo-ACP synthase -
BVMH_RS02470 483666..484673 + 1008 WP_007410230.1 outer membrane lipoprotein carrier protein LolA -
BVMH_RS02475 484790..485959 + 1170 WP_025284272.1 alanine racemase -
BVMH_RS02480 486079..486360 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
BVMH_RS02485 486366..486716 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
BVMH_RS02490 486834..487655 + 822 WP_003156182.1 STAS domain-containing protein -
BVMH_RS02495 487660..488025 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
BVMH_RS02500 488028..488429 + 402 WP_003156178.1 anti-sigma regulatory factor -
BVMH_RS02505 488441..489448 + 1008 WP_007410233.1 PP2C family protein-serine/threonine phosphatase -
BVMH_RS02510 489512..489841 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
BVMH_RS02515 489838..490320 + 483 WP_007609591.1 anti-sigma B factor RsbW -
BVMH_RS02520 490286..491074 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
BVMH_RS02525 491074..491676 + 603 WP_007410234.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T115453 WP_003156187.1 NZ_CP034176:486366-486716 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T115453 NZ_CP034176:486366-486716 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT115453 WP_003156188.1 NZ_CP034176:486079-486360 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT115453 NZ_CP034176:486079-486360 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References