Detailed information of TA system TA115450

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 870323..870894
• Replicon: chromosome
• Accession: NZ_CP034169
• Organism: Enterococcus avium strain 352

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: EH197_RS04750
• Protein ID: WP_016178972.1
• Coordinates: 870323..870664 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A6I7Z7X1
• Locus tag: EH197_RS04755
• Protein ID: WP_016178973.1
• Coordinates: 870664..870894 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EH197_RS04720	866008..866256	+	249	WP_051122613.1	hypothetical protein	-
EH197_RS04725	866733..867374	+	642	WP_016178968.1	hypothetical protein	-
EH197_RS04730	867455..868066	+	612	WP_016178969.1	helix-turn-helix domain-containing protein	-
EH197_RS04735	868192..868476	+	285	WP_016178970.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
EH197_RS04740	868469..868760	+	292	Protein_860	type II toxin-antitoxin system YafQ family toxin	-
EH197_RS04745	868935..870128	+	1194	WP_016178971.1	arginine deiminase	-
EH197_RS04750	870323..870664	-	342	WP_016178972.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
EH197_RS04755	870664..870894	-	231	WP_016178973.1	hypothetical protein	Antitoxin
EH197_RS04760	871067..871675	+	609	WP_016178974.1	Fic family protein	-
EH197_RS04765	872049..872468	+	420	WP_016178975.1	hypothetical protein	-
EH197_RS04770	872493..874553	+	2061	WP_070503443.1	glucose-specific PTS transporter subunit IIBC	-
EH197_RS04775	874573..875550	+	978	WP_070503445.1	ROK family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13160.42 Da
• Isoelectric Point: 9.7082

>T115450 WP_016178972.1 NZ_CP034169:c870664-870323 [Enterococcus avium]
MTEEKIYVPKKGDIVWIDFDPSAGKEIQKRRPGLVVSRYEFNRKTLFAVICPITSTIRNLPTRYSLPNDLDTTGQILISQ
LKSLDFKERKLKKIESLPLQDMAKIDQIIEYIF

Nucleotide

• Length: 342 bp

>T115450 NZ_CP034169:c870664-870323 [Enterococcus avium]
ATGACTGAAGAAAAAATCTATGTTCCTAAAAAAGGAGATATTGTGTGGATTGATTTTGATCCTTCCGCAGGAAAAGAGAT
TCAAAAGAGACGTCCTGGGTTGGTCGTATCTAGATACGAGTTTAACCGTAAAACGCTGTTTGCAGTGATTTGCCCGATTA
CTTCTACAATAAGAAACTTACCAACACGTTATTCTTTACCAAACGATTTAGACACTACGGGACAAATACTTATTTCTCAA
TTAAAATCACTTGATTTCAAAGAGAGAAAGCTCAAAAAAATTGAATCCTTACCTTTGCAAGATATGGCTAAAATAGATCA
AATTATTGAATATATATTTTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8520.52 Da
• Isoelectric Point: 4.1597

>AT115450 WP_016178973.1 NZ_CP034169:c870894-870664 [Enterococcus avium]
MLIAKSRLQGSSVVITLPSDNGKKPKENKEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWTDLSPEGREIL

Nucleotide

• Length: 231 bp

>AT115450 NZ_CP034169:c870894-870664 [Enterococcus avium]
ATGCTTATTGCAAAATCTAGATTACAAGGAAGCTCTGTAGTGATAACCTTACCTTCGGATAACGGGAAGAAACCTAAAGA
AAACAAAGAGTATATTGTTGTCTATTCTGATGATGGGACAATCACATTAGTTCCCAAAATCGAAGATCCCTTCAGTGGTG
GAGAAGAAGCGGAGTATTATGAAAAAGACGAATGGACAGATTTATCTCCTGAAGGAAGAGAGATTTTATAA

Structures

Antitoxin

• AlphaFold DB: A0A6I7Z7X1