Detailed information of TA system TA115448

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 99828..100471
• Replicon: plasmid p2014C-4015-3
• Accession: NZ_CP034167
• Organism: Escherichia albertii strain 2014C-4015

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: AYR02_RS24440
• Protein ID: WP_124783386.1
• Coordinates: 99828..100244 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A5Z7X8E3
• Locus tag: AYR02_RS24445
• Protein ID: WP_001644959.1
• Coordinates: 100241..100471 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AYR02_RS24420	95900..96439	+	540	Protein_103	fimbrial protein	-
AYR02_RS24425	96752..97108	+	357	WP_124783402.1	hypothetical protein	-
AYR02_RS24760	97451..97975	+	525	WP_164474009.1	hypothetical protein	-
AYR02_RS24430	97956..99124	+	1169	WP_124781951.1	IS3-like element IS911 family transposase	-
AYR02_RS24435	99130..99672	-	543	WP_124783385.1	class I SAM-dependent methyltransferase	-
AYR02_RS24440	99828..100244	-	417	WP_124783386.1	type II toxin-antitoxin system VapC family toxin	Toxin
AYR02_RS24445	100241..100471	-	231	WP_001644959.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
AYR02_RS24455	100967..101506	+	540	WP_124783387.1	hypothetical protein	-
AYR02_RS24460	101639..101787	-	149	Protein_111	IS110 family transposase	-
AYR02_RS24465	102334..103284	+	951	WP_124783388.1	MBL fold metallo-hydrolase	-
AYR02_RS24470	103288..103605	+	318	WP_059236668.1	hypothetical protein	-
AYR02_RS24475	103593..104444	+	852	WP_124783389.1	DUF692 domain-containing protein	-
AYR02_RS24480	104419..105132	+	714	WP_164474010.1	putative DNA-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	espL2 / vat / papC / papD	1..136645	136645

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-50)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15051.37 Da
• Isoelectric Point: 7.2447

>T115448 WP_124783386.1 NZ_CP034167:c100244-99828 [Escherichia albertii]
VNKTYMLDTNICSFIMREQPEAVIRRLEQAVLRNHRIVVSAITYAEMRFGAIGKKASPRHGQLVDAFCARLDAILPWDRA
AVDATVEVKAALTAAGTPIGPNDTAIAGHAIAAGAILVTNNTREFEQVPNLVLEDWVK

Nucleotide

• Length: 417 bp

>T115448 NZ_CP034167:c100244-99828 [Escherichia albertii]
GTGAACAAAACGTATATGCTGGATACTAATATCTGCTCATTCATCATGCGTGAGCAGCCGGAGGCCGTGATCAGACGCTT
AGAGCAAGCGGTGCTGCGCAACCATCGCATCGTGGTCTCCGCTATTACCTACGCCGAAATGCGCTTCGGTGCTATCGGGA
AAAAAGCCTCGCCCCGTCATGGTCAACTGGTCGATGCGTTCTGCGCCCGCCTCGATGCGATTCTGCCGTGGGACCGTGCC
GCAGTCGATGCCACTGTAGAGGTCAAAGCAGCACTAACCGCAGCCGGTACACCAATCGGTCCCAACGACACAGCGATTGC
CGGACATGCTATAGCTGCAGGGGCCATTCTGGTGACCAATAATACGCGAGAGTTTGAGCAGGTGCCAAATTTGGTATTAG
AAGACTGGGTTAAATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8706.80 Da
• Isoelectric Point: 4.4692

>AT115448 WP_001644959.1 NZ_CP034167:c100471-100241 [Escherichia albertii]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQLEKADADFLVEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT115448 NZ_CP034167:c100471-100241 [Escherichia albertii]
ATGCGAACAGTATCTATTTTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCTCGCGATCTGGACTTTGAGGGGGTGAG
TGAGTTGGAGATTGTCCGCGAAGGGGACAGCATCATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
AAAAGGCCGACGCAGACTTTTTGGTGGAGCGTGAAGACGTGGTCAGTGACGAAGGACGATTTAACCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A5Z7X8E3