Detailed information of TA system TA115446

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 52941..53566
• Replicon: plasmid p2014C-4015-3
• Accession: NZ_CP034167
• Organism: Escherichia albertii strain 2014C-4015

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: AYR02_RS24200
• Protein ID: WP_059278837.1
• Coordinates: 52941..53339 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: AYR02_RS24205
• Protein ID: WP_164474007.1
• Coordinates: 53339..53566 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AYR02_RS24185	49206..49727	+	522	WP_124783363.1	conjugal transfer entry exclusion protein TraS	-
AYR02_RS24190	49769..50482	+	714	WP_000989337.1	complement resistance protein TraT	-
AYR02_RS24195	50734..52932	+	2199	WP_124783364.1	type IV conjugative transfer system coupling protein TraD	-
AYR02_RS24200	52941..53339	-	399	WP_059278837.1	tRNA(fMet)-specific endonuclease VapC	Toxin
AYR02_RS24205	53339..53566	-	228	WP_164474007.1	toxin-antitoxin system antitoxin VapB	Antitoxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	espL2 / vat / papC / papD	1..136645	136645

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14898.11 Da
• Isoelectric Point: 7.2453

>T115446 WP_059278837.1 NZ_CP034167:c53339-52941 [Escherichia albertii]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHT
GQIRAELSHQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVDGLRIEDWS

Nucleotide

• Length: 399 bp

>T115446 NZ_CP034167:c53339-52941 [Escherichia albertii]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTTACGATAAAGAACAAACCCGCCAGTGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGCTCTGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGCAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCTGCAGCCACACACACC
GGCCAGATAAGAGCAGAACTTTCCCATCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGGCTGATAGTTGTAACTAACAACACCCGGGAATTTGAGCGTGTGGACGGCCTGAGAATTGAAGACTGGAGCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8484.50 Da
• Isoelectric Point: 4.5358

>AT115446 WP_164474007.1 NZ_CP034167:c53566-53339 [Escherichia albertii]
METSVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGESWDEWFDGNSVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT115446 NZ_CP034167:c53566-53339 [Escherichia albertii]
ATGGAAACCAGCGTATTTCTCAGTAACCGCAGCCAGGCGGTCAGGCTGCCAAAAGCAGTTGCCCTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCCGTATCATCACGCCAGCCGGAGAATCCTGGGATGAATGGTTCGACGGAA
ACAGTGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA