Detailed information of TA system TA115445

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 9275..9810
• Replicon: plasmid p2014C-4015-3
• Accession: NZ_CP034167
• Organism: Escherichia albertii strain 2014C-4015

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A8H9KAX0
• Locus tag: AYR02_RS23935
• Protein ID: WP_000222759.1
• Coordinates: 9523..9810 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A8H9KB11
• Locus tag: AYR02_RS23930
• Protein ID: WP_001132891.1
• Coordinates: 9275..9526 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AYR02_RS23905	4566..5543	-	978	WP_059278929.1	RepB family plasmid replication initiator protein	-
AYR02_RS23920	6624..7790	+	1167	WP_059278927.1	plasmid-partitioning protein SopA	-
AYR02_RS23925	7790..8761	+	972	WP_124783341.1	ParB/RepB/Spo0J family plasmid partition protein	-
AYR02_RS23930	9275..9526	+	252	WP_001132891.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
AYR02_RS23935	9523..9810	+	288	WP_000222759.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AYR02_RS24895	9946..10842	+	897	Protein_11	transposase	-
AYR02_RS23950	11145..11858	-	714	WP_059278925.1	molecular chaperone	-
AYR02_RS23955	11855..13183	-	1329	WP_124783343.1	fimbrial protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	espL2 / vat / papC / papD	1..136645	136645
	-	flank	IS/Tn	-	-	10487..10603	116

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11176.23 Da
• Isoelectric Point: 10.5832

>T115445 WP_000222759.1 NZ_CP034167:9523-9810 [Escherichia albertii]
MTYTVKFRDDALKEWLKLDKSIQQQFAKKLKKCSENPHIPSAKLRELKNCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYNLASERMR

Nucleotide

• Length: 288 bp

>T115445 NZ_CP034167:9523-9810 [Escherichia albertii]
ATGACTTATACGGTAAAATTCAGGGATGATGCGCTCAAAGAATGGCTGAAACTGGATAAATCCATTCAGCAACAGTTCGC
AAAAAAACTAAAAAAATGCAGCGAAAATCCTCATATACCTTCAGCAAAACTACGTGAACTAAAAAATTGCTACAAAATCA
AACTTCGTGCATCGGGTTTTCGTCTGGTCTATCAGGTTATTGATGACATGTTAATTATTGCGGTTGTCGCAGTAGGTAAA
CGTGAACGTAGTAACGTTTATAATCTTGCCAGCGAGAGAATGAGATAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9181.39 Da
• Isoelectric Point: 4.0780

>AT115445 WP_001132891.1 NZ_CP034167:9275-9526 [Escherichia albertii]
MPNIILSDTSASVSELKKNPMATVGAGDGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSNQPLHDVDLD
SYL

Nucleotide

• Length: 252 bp

>AT115445 NZ_CP034167:9275-9526 [Escherichia albertii]
ATGCCTAATATCATTCTGTCTGACACCAGTGCAAGTGTCAGTGAACTGAAAAAAAACCCAATGGCAACAGTCGGTGCCGG
AGATGGTTATCCTGTCGCCATTCTTAACCGAAACCAGCCGGCATTCTACTGTGTGCCTGCGGAACTGTATGAAAGGATGC
TTGATGCTCTGGATGATCAGGAGCTGGTGAAACTGGTTACTGAACGCAGCAATCAGCCACTGCATGATGTGGACCTGGAT
AGTTATTTATGA