Detailed information of TA system TA115441

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 4274705..4275176
• Replicon: chromosome
• Accession: NZ_CP034166
• Organism: Escherichia albertii strain 2014C-4015

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0D7C2L1
• Locus tag: AYR02_RS21975
• Protein ID: WP_001303511.1
• Coordinates: 4274705..4274983 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XAD5
• Locus tag: AYR02_RS21980
• Protein ID: WP_001302048.1
• Coordinates: 4274985..4275176 (-)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AYR02_RS21940	4270046..4270297	-	252	WP_000273158.1	DUF4224 domain-containing protein	-
AYR02_RS21945	4270364..4272844	-	2481	WP_124783177.1	exonuclease	-
AYR02_RS21950	4272925..4273128	-	204	WP_059219682.1	DUF1482 family protein	-
AYR02_RS21955	4273125..4273313	-	189	WP_124783178.1	cell division inhibition protein DicB	-
AYR02_RS21965	4273879..4274097	-	219	WP_001171923.1	hypothetical protein	-
AYR02_RS21970	4274257..4274412	-	156	WP_072248456.1	DUF1391 family protein	-
AYR02_RS21975	4274705..4274983	-	279	WP_001303511.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AYR02_RS21980	4274985..4275176	-	192	WP_001302048.1	hypothetical protein	Antitoxin
AYR02_RS21985	4275197..4275568	-	372	WP_001169685.1	hypothetical protein	-
AYR02_RS21990	4275666..4275968	+	303	WP_000172735.1	transcriptional regulator	-
AYR02_RS21995	4275965..4276390	+	426	Protein_4004	Rha family transcriptional regulator	-
AYR02_RS22000	4276462..4277539	+	1078	Protein_4005	phage replisome organizer	-
AYR02_RS22005	4277580..4278001	+	422	Protein_4006	DUF977 family protein	-
AYR02_RS22010	4278059..4278415	+	357	WP_021527610.1	hypothetical protein	-
AYR02_RS22015	4278509..4278691	+	183	WP_059278956.1	hypothetical protein	-
AYR02_RS24730	4278684..4278860	+	177	WP_000753060.1	hypothetical protein	-
AYR02_RS22020	4278857..4279184	+	328	Protein_4010	hypothetical protein	-
AYR02_RS22035	4279915..4280070	+	156	WP_113649980.1	type I toxin-antitoxin system toxin HokD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4268045..4293269	25224

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10661.25 Da
• Isoelectric Point: 5.5647

>T115441 WP_001303511.1 NZ_CP034166:c4274983-4274705 [Escherichia albertii]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Nucleotide

• Length: 279 bp

>T115441 NZ_CP034166:c4274983-4274705 [Escherichia albertii]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7463.47 Da
• Isoelectric Point: 8.6828

>AT115441 WP_001302048.1 NZ_CP034166:c4275176-4274985 [Escherichia albertii]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Nucleotide

• Length: 192 bp

>AT115441 NZ_CP034166:c4275176-4274985 [Escherichia albertii]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGCGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAAATGCGCAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• PDB: 5CZF
• PDB: 5CW7
• PDB: 5CZE

Antitoxin

• PDB: 5CZF
• PDB: 3ZBE
• PDB: 5CW7
• AlphaFold DB: A0A829CTP0