Detailed information of TA system TA115413

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataT-KacA/DUF1778(antitoxin)
• Location: 1724..2522
• Replicon: plasmid p2014C-4015-1
• Accession: NZ_CP034165
• Organism: Escherichia albertii strain 2014C-4015

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: AYR02_RS00605
• Protein ID: WP_124781934.1
• Coordinates: 1724..2245 (-)
• Length: 174 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: A0A0V9R6Q7
• Locus tag: AYR02_RS00610
• Protein ID: WP_001351987.1
• Coordinates: 2253..2522 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AYR02_RS00590	968..1303	+	336	Protein_1	hypothetical protein	-
AYR02_RS00595	1305..1556	+	252	WP_001404395.1	hypothetical protein	-
AYR02_RS00605	1724..2245	-	522	WP_124781934.1	GNAT family N-acetyltransferase	Toxin
AYR02_RS00610	2253..2522	-	270	WP_001351987.1	DUF1778 domain-containing protein	Antitoxin
AYR02_RS00615	2841..3509	+	669	WP_000161228.1	AAA family ATPase	-
AYR02_RS00620	3515..3868	+	354	WP_160378290.1	hypothetical protein	-
AYR02_RS00625	3921..4688	-	768	WP_000342417.1	hypothetical protein	-
AYR02_RS00630	4973..5713	+	741	WP_001717320.1	hypothetical protein	-
AYR02_RS00635	5757..7094	+	1338	WP_124781935.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..63807	63807
	-	flank	IS/Tn	-	-	592..894	302

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (80-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 174 a.a.
• Molecular weight: 19475.31 Da
• Isoelectric Point: 8.6344

>T115413 WP_124781934.1 NZ_CP034165:c2245-1724 [Escherichia albertii]
VDNIKIEIFSGEKDYDLNGFDCGEESLNAFLTNHLKRQHEGKILRAYVLCTKEDRPKVLGYYTLSGSCFEKESLPSRSQQ
KKVPYRNVPSVTLGRLALDKSLQGQGFGSMLVTHAMRVVYNASLAVGIHGLFVEALNDKAKAFYKSLGFIQLVGNNERSL
FYPTKSIEKLFEE

Nucleotide

• Length: 522 bp

>T115413 NZ_CP034165:c2245-1724 [Escherichia albertii]
GTGGATAATATAAAGATAGAAATTTTCTCTGGAGAGAAAGATTATGATTTGAATGGTTTTGATTGCGGTGAAGAATCACT
AAATGCCTTTTTAACCAACCACTTAAAAAGGCAGCATGAGGGAAAAATTCTTCGCGCTTATGTGCTTTGCACTAAAGAAG
ACAGACCAAAAGTGTTAGGGTATTACACTTTGTCAGGCAGCTGCTTTGAAAAGGAGTCCTTACCTTCAAGAAGCCAGCAA
AAGAAGGTACCTTATCGGAATGTTCCAAGTGTTACTTTGGGTAGGCTGGCTTTGGACAAATCTCTTCAAGGTCAAGGATT
TGGCTCAATGCTTGTAACACATGCAATGCGCGTTGTGTACAATGCATCTCTTGCTGTAGGCATTCATGGACTTTTCGTTG
AGGCGTTAAATGACAAAGCCAAGGCGTTTTATAAAAGTTTAGGCTTTATCCAGCTGGTTGGTAACAACGAGCGTTCTTTG
TTCTATCCTACAAAATCTATCGAAAAATTGTTCGAAGAATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10082.54 Da
• Isoelectric Point: 7.0155

>AT115413 WP_001351987.1 NZ_CP034165:c2522-2253 [Escherichia albertii]
MSALKKQRIDLRLTDDDKSIIEEAAAMSNQSITQFMVSSASERAVKVIEQHRRLVLNEESWNLVMDAISNPPAPNDKLKR
AADRLKSME

Nucleotide

• Length: 270 bp

>AT115413 NZ_CP034165:c2522-2253 [Escherichia albertii]
ATGTCAGCACTAAAAAAGCAGCGCATCGATTTGAGATTAACCGATGACGATAAAAGCATCATCGAGGAAGCTGCCGCAAT
GTCTAACCAGAGCATTACTCAGTTTATGGTTAGCAGTGCATCTGAACGTGCCGTGAAAGTTATAGAGCAACACCGTAGAC
TAGTTCTGAATGAAGAATCCTGGAATCTGGTTATGGACGCTATAAGCAATCCTCCGGCACCGAACGACAAGCTGAAACGA
GCTGCTGATCGTCTGAAAAGCATGGAGTAG

Structures

Antitoxin

• AlphaFold DB: A0A0V9R6Q7