Detailed information of TA system TA115411

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 77567..78168
• Replicon: plasmid p06-3542
• Accession: NZ_CP034163
• Organism: Escherichia albertii strain 06-3542

Toxin (Protein)

• Gene name: doc
• Uniprot ID: V0AJ64
• Locus tag: CCS91_RS23775
• Protein ID: WP_001216034.1
• Coordinates: 77567..77947 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: CCS91_RS23780
• Protein ID: WP_086351776.1
• Coordinates: 77947..78168 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCS91_RS23750	73243..74436	-	1194	WP_124866781.1	terminase	-
CCS91_RS23755	74502..75713	-	1212	WP_124866782.1	transposase	-
CCS91_RS23760	75756..76223	-	468	WP_124866783.1	Late promoter-activating protein	-
CCS91_RS23765	76312..77355	-	1044	WP_124866784.1	DUF968 domain-containing protein	-
CCS91_RS23770	77383..77562	-	180	WP_024262170.1	hypothetical protein	-
CCS91_RS23775	77567..77947	-	381	WP_001216034.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
CCS91_RS23780	77947..78168	-	222	WP_086351776.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
CCS91_RS23785	78313..78777	-	465	WP_124866785.1	hypothetical protein	-
CCS91_RS23790	78862..79497	-	636	WP_124866786.1	hypothetical protein	-
CCS91_RS24090	79712..79888	-	177	WP_164473978.1	hypothetical protein	-
CCS91_RS23800	79888..80181	-	294	WP_124866788.1	hypothetical protein	-
CCS91_RS23805	80360..80593	-	234	WP_124866789.1	hypothetical protein	-
CCS91_RS23810	80676..81659	-	984	WP_124866790.1	DUF551 domain-containing protein	-
CCS91_RS23815	81670..81867	-	198	WP_000360278.1	hypothetical protein	-
CCS91_RS23820	81869..82318	-	450	WP_124866791.1	ead/Ea22-like family protein	-
CCS91_RS23825	82306..82951	-	646	Protein_91	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..95683	95683
	-	flank	IS/Tn	-	-	74502..75653	1151

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (2-123)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13615.32 Da
• Isoelectric Point: 5.1514

>T115411 WP_001216034.1 NZ_CP034163:c77947-77567 [Escherichia albertii]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T115411 NZ_CP034163:c77947-77567 [Escherichia albertii]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCAACCTACCTGGTGGCTACTGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTAACCGTAGGTGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCCGCGGAGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8160.12 Da
• Isoelectric Point: 4.7708

>AT115411 WP_086351776.1 NZ_CP034163:c78168-77947 [Escherichia albertii]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVNKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT115411 NZ_CP034163:c78168-77947 [Escherichia albertii]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAACAAGGCTACTTTTGAAGCCTACAAAAAAGCGGCGCTGGATG
CCGAATTTGCATCCCTGTTTGACACTCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Toxin

• AlphaFold DB: V0AJ64