Detailed information of TA system TA115389

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 427888..428423
• Replicon: plasmid unnamed4
• Accession: NZ_CP034156
• Organism: Rhodococcus sp. NJ-530

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: EHW12_RS33820
• Protein ID: WP_064444974.1
• Coordinates: 427888..428187 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A3G9AJN8
• Locus tag: EHW12_RS33825
• Protein ID: WP_042445813.1
• Coordinates: 428187..428423 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EHW12_RS33795	422906..423319	-	414	WP_003941273.1	hypothetical protein	-
EHW12_RS33800	423429..423752	-	324	Protein_440	cutinase	-
EHW12_RS33805	424012..425082	+	1071	WP_125053520.1	DUF1016 family protein	-
EHW12_RS33810	425212..426009	-	798	WP_125053522.1	hypothetical protein	-
EHW12_RS33815	426009..427859	-	1851	WP_125053524.1	hypothetical protein	-
EHW12_RS33820	427888..428187	-	300	WP_064444974.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
EHW12_RS33825	428187..428423	-	237	WP_042445813.1	hypothetical protein	Antitoxin
EHW12_RS33830	428796..429536	-	741	WP_082920770.1	TetR/AcrR family transcriptional regulator	-
EHW12_RS33835	429533..430501	-	969	WP_064444976.1	DUF2637 domain-containing protein	-
EHW12_RS33840	431132..431446	-	315	WP_064444977.1	hypothetical protein	-
EHW12_RS33845	431443..432225	-	783	WP_029256883.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..565367	565367

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (11-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 10440.96 Da
• Isoelectric Point: 4.4988

>T115389 WP_064444974.1 NZ_CP034156:c428187-427888 [Rhodococcus sp. NJ-530]
MRPIHEADLDTVRPVLVLTRQVVRPHLGTVTVAPITDEIRGLSTEVSVSAANGLSADAVVSCDHITTIPVSALGNVIGYL
LPAQEESLSIAVRAAFDLE

Nucleotide

• Length: 300 bp

>T115389 NZ_CP034156:c428187-427888 [Rhodococcus sp. NJ-530]
ATGCGACCAATTCACGAAGCCGACCTCGACACAGTCCGCCCGGTCCTCGTACTCACCCGACAGGTGGTCCGTCCACACCT
CGGGACCGTCACGGTAGCGCCCATCACCGACGAGATCCGAGGACTGTCCACTGAGGTGTCCGTCAGTGCCGCGAACGGAC
TATCCGCCGACGCGGTGGTCAGTTGCGATCACATCACCACCATTCCCGTCAGTGCACTCGGCAACGTCATCGGTTATCTT
CTGCCAGCACAAGAAGAATCACTGTCCATAGCCGTGCGCGCCGCATTCGATCTCGAATGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8633.62 Da
• Isoelectric Point: 6.2181

>AT115389 WP_042445813.1 NZ_CP034156:c428423-428187 [Rhodococcus sp. NJ-530]
MSTQIAVRLPDEIVAFLDDEIREQRATSRASVVLRALERERRRQIAARDAEILANATSEHDLSSLAHHAANPQSTGLS

Nucleotide

• Length: 237 bp

>AT115389 NZ_CP034156:c428423-428187 [Rhodococcus sp. NJ-530]
ATGAGCACGCAGATCGCGGTACGACTACCGGACGAGATCGTGGCATTTCTCGACGACGAAATCAGAGAACAGCGCGCCAC
CTCGCGAGCCTCGGTAGTGTTGCGCGCTCTCGAACGCGAGCGTCGCCGACAGATCGCCGCACGTGACGCGGAGATTCTCG
CGAACGCCACATCCGAACATGACCTGAGTTCTCTCGCTCACCACGCCGCCAATCCGCAGTCAACAGGCCTGAGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A3G9AJN8