Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 400680..401245 Replicon plasmid unnamed4
Accession NZ_CP034156
Organism Rhodococcus sp. NJ-530

Toxin (Protein)


Gene name mazF Uniprot ID C3JNM9
Locus tag EHW12_RS33680 Protein ID WP_003943396.1
Coordinates 400680..401036 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID C3JNM8
Locus tag EHW12_RS33685 Protein ID WP_003943374.1
Coordinates 401033..401245 (-) Length 71 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EHW12_RS33660 397038..397478 + 441 WP_024488231.1 hypothetical protein -
EHW12_RS33670 398558..399070 + 513 WP_064442835.1 hypothetical protein -
EHW12_RS33675 400093..400482 + 390 WP_007728194.1 helix-turn-helix domain-containing protein -
EHW12_RS33680 400680..401036 - 357 WP_003943396.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
EHW12_RS33685 401033..401245 - 213 WP_003943374.1 ribbon-helix-helix domain-containing protein Antitoxin
EHW12_RS33695 402549..403582 + 1034 Protein_419 diiron oxygenase -
EHW12_RS33700 403793..404491 - 699 WP_125053503.1 IS6 family transposase -
EHW12_RS33705 404809..405899 + 1091 Protein_421 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..565367 565367


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-117)

Antitoxin

Ribbon-helix-helix protein, CopG

(5-39)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12844.58 Da        Isoelectric Point: 6.8817

>T115388 WP_003943396.1 NZ_CP034156:c401036-400680 [Rhodococcus sp. NJ-530]
MIRRAHIHWVDLDPAQGSEAAKRRPAVVVSNDRANATAENLGRGVITVVPLTTNTAHVFPFQVIVESDESGLARASKAQA
EQVRSVSVRRLDPEPVGKVGMETFAALEDALRLHLDLQ

Download         Length: 357 bp

>T115388 NZ_CP034156:c401036-400680 [Rhodococcus sp. NJ-530]
ATGATTCGCCGCGCGCACATTCACTGGGTGGATCTCGATCCGGCGCAGGGCAGTGAAGCGGCGAAACGACGACCGGCGGT
GGTGGTGAGCAACGACCGGGCCAACGCTACTGCAGAAAATCTCGGCAGGGGCGTGATCACCGTGGTTCCGTTGACTACCA
ATACCGCGCACGTGTTTCCGTTTCAGGTGATTGTCGAGTCCGACGAGTCAGGTTTGGCGCGAGCGTCGAAAGCGCAAGCG
GAGCAAGTGCGTTCGGTATCGGTTCGGCGTCTGGATCCTGAACCTGTCGGCAAGGTCGGGATGGAGACTTTCGCCGCACT
CGAGGACGCGCTGCGGCTGCACCTCGATCTGCAGTAA

Antitoxin


Download         Length: 71 a.a.        Molecular weight: 7682.43 Da        Isoelectric Point: 4.1824

>AT115388 WP_003943374.1 NZ_CP034156:c401245-401033 [Rhodococcus sp. NJ-530]
MTTKFTISLPDEDAHLLDEYVRDHGLRGRSAGIRAALRLLSTSGLERDYAAAFAEGDVDEVWDAVTADTE

Download         Length: 213 bp

>AT115388 NZ_CP034156:c401245-401033 [Rhodococcus sp. NJ-530]
ATGACCACGAAATTTACCATAAGTCTGCCTGACGAGGATGCGCACCTACTCGACGAGTATGTGCGTGATCACGGACTTCG
TGGCCGGTCGGCAGGAATTCGAGCCGCACTCCGCCTACTCTCGACGTCCGGACTCGAACGTGACTACGCTGCAGCGTTTG
CTGAAGGTGACGTAGATGAAGTGTGGGACGCTGTCACGGCAGACACCGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2PUJ5

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2PWJ9

References