Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RelE-Phd
Location 296742..297304 Replicon plasmid unnamed4
Accession NZ_CP034156
Organism Rhodococcus sp. NJ-530

Toxin (Protein)


Gene name relE Uniprot ID A0A3G9A8R6
Locus tag EHW12_RS33150 Protein ID WP_054828169.1
Coordinates 296742..297032 (-) Length 97 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A3G9A8Q8
Locus tag EHW12_RS33155 Protein ID WP_054828168.1
Coordinates 297029..297304 (-) Length 92 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EHW12_RS33125 291950..292234 - 285 WP_125053390.1 helix-turn-helix transcriptional regulator -
EHW12_RS33130 292231..293310 - 1080 WP_075835920.1 tyrosine-type recombinase/integrase -
EHW12_RS33135 293435..293722 - 288 WP_125053392.1 glutaredoxin-like protein NrdH -
EHW12_RS33140 294333..295325 + 993 WP_125053394.1 DUF2971 domain-containing protein -
EHW12_RS33145 295322..296605 + 1284 WP_125053396.1 IS256 family transposase -
EHW12_RS33150 296742..297032 - 291 WP_054828169.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
EHW12_RS33155 297029..297304 - 276 WP_054828168.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
EHW12_RS33160 297811..298875 - 1065 WP_125053398.1 ATP-dependent Clp protease ATP-binding subunit -
EHW12_RS33165 299001..300038 + 1038 WP_125053400.1 aliphatic amidase -
EHW12_RS33170 300198..301277 + 1080 WP_054828167.1 substrate-binding domain-containing protein -
EHW12_RS33175 301301..301645 - 345 WP_095972697.1 MarR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..565367 565367


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(14-94)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 97 a.a.        Molecular weight: 10949.34 Da        Isoelectric Point: 7.5715

>T115387 WP_054828169.1 NZ_CP034156:c297032-296742 [Rhodococcus sp. NJ-530]
MSDAPTDSSDPYRVEVASPARRDLQRLPSRIVHAVIEFISGPLAENPHRLSKPLRDDLDGLHSARRGDYRILLHIDDPNH
TIVIVRIDHRAHAYRT

Download         Length: 291 bp

>T115387 NZ_CP034156:c297032-296742 [Rhodococcus sp. NJ-530]
ATGAGCGACGCTCCCACAGACTCGTCCGACCCGTACCGGGTCGAAGTCGCATCCCCAGCTCGCCGCGACCTTCAACGTCT
CCCATCCCGGATCGTCCACGCCGTCATCGAATTCATCTCCGGACCCCTCGCCGAGAACCCTCACCGGCTCAGCAAACCGC
TACGCGACGACCTCGACGGTCTCCACAGCGCCCGCCGCGGCGACTACCGAATCCTGCTCCACATCGACGACCCCAACCAC
ACCATCGTCATCGTCAGAATCGACCACCGTGCCCACGCCTATCGCACCTAG

Antitoxin


Download         Length: 92 a.a.        Molecular weight: 9694.82 Da        Isoelectric Point: 4.6221

>AT115387 WP_054828168.1 NZ_CP034156:c297304-297029 [Rhodococcus sp. NJ-530]
MTTVPLGEAKDKLSALVDSAETTHDIITITKHGKPAAVLMSADDLESLHETIYWLSRAGVRDDVATADAEYAAGHTASID
DLRAEHGLPPK

Download         Length: 276 bp

>AT115387 NZ_CP034156:c297304-297029 [Rhodococcus sp. NJ-530]
ATGACGACAGTGCCACTGGGTGAAGCCAAGGACAAGCTCTCCGCGCTGGTCGATAGCGCCGAAACCACGCACGACATCAT
CACGATCACCAAGCACGGCAAACCCGCCGCGGTGCTGATGTCTGCCGACGACCTCGAATCGCTGCACGAGACGATCTACT
GGCTCTCACGTGCCGGTGTCCGCGACGACGTCGCTACCGCCGACGCCGAGTACGCTGCCGGCCACACTGCCTCCATCGAC
GACCTGCGCGCGGAACACGGCCTGCCGCCGAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3G9A8R6

Antitoxin

Source ID Structure
AlphaFold DB A0A3G9A8Q8

References