Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 106992..107510 Replicon plasmid unnamed4
Accession NZ_CP034156
Organism Rhodococcus sp. NJ-530

Toxin (Protein)


Gene name mazF Uniprot ID A0A3G9AIV5
Locus tag EHW12_RS32070 Protein ID WP_064444829.1
Coordinates 106992..107294 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID C3JHS6
Locus tag EHW12_RS32075 Protein ID WP_003941254.1
Coordinates 107295..107510 (-) Length 72 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EHW12_RS32060 104203..104999 - 797 Protein_98 hypothetical protein -
EHW12_RS32065 104999..106396 - 1398 WP_125053623.1 hypothetical protein -
EHW12_RS32070 106992..107294 - 303 WP_064444829.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
EHW12_RS32075 107295..107510 - 216 WP_003941254.1 hypothetical protein Antitoxin
EHW12_RS34855 107709..108002 - 294 WP_164490658.1 hypothetical protein -
EHW12_RS32080 108032..109327 - 1296 WP_125053200.1 AAA family ATPase -
EHW12_RS32085 109398..110585 - 1188 WP_125053202.1 hypothetical protein -
EHW12_RS32090 110582..110776 - 195 WP_125053203.1 hypothetical protein -
EHW12_RS32095 110773..112011 - 1239 WP_125053205.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..565367 565367


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-93)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 10513.08 Da        Isoelectric Point: 5.0492

>T115386 WP_064444829.1 NZ_CP034156:c107294-106992 [Rhodococcus sp. NJ-530]
VRPIHEAQLGDLCPVLVLTRELVRPHLGTVTVAPIRSKGRGLSTELSLGEGNGVEDGSVVDCDGVTTIPVSALGVNRGFL
LPAQEVLLSRALRSAFDLES

Download         Length: 303 bp

>T115386 NZ_CP034156:c107294-106992 [Rhodococcus sp. NJ-530]
GTGCGCCCGATCCACGAGGCGCAACTCGGAGATCTTTGTCCGGTGTTGGTCCTGACGAGGGAATTGGTTCGGCCCCACCT
GGGGACGGTCACTGTTGCCCCCATTCGTTCGAAGGGGCGGGGGTTGTCGACCGAACTGAGTTTAGGTGAAGGCAACGGCG
TCGAGGACGGATCGGTTGTCGACTGCGACGGGGTGACGACCATCCCCGTGTCGGCTCTCGGGGTGAATCGAGGTTTTCTC
TTGCCGGCTCAGGAAGTGTTGTTGAGCAGGGCGTTGCGATCAGCATTTGATCTCGAGTCATGA

Antitoxin


Download         Length: 72 a.a.        Molecular weight: 7670.69 Da        Isoelectric Point: 8.9167

>AT115386 WP_003941254.1 NZ_CP034156:c107510-107295 [Rhodococcus sp. NJ-530]
MSTQIAVRLPEDIVAFLDSEVSGGRAKSRAQVVLRALEREQRRQLAVRDVEILSDGAGSDVNIAAAHGKKN

Download         Length: 216 bp

>AT115386 NZ_CP034156:c107510-107295 [Rhodococcus sp. NJ-530]
ATGAGTACGCAGATCGCGGTACGGCTACCCGAGGACATCGTGGCGTTTCTCGACAGCGAGGTCAGCGGTGGGCGTGCGAA
AAGCCGTGCTCAAGTCGTGCTTCGAGCGTTGGAGCGTGAGCAGCGTCGACAGTTGGCGGTCCGCGACGTGGAGATCCTGT
CTGACGGCGCGGGTAGCGATGTGAATATCGCTGCCGCGCACGGGAAGAAGAACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3G9AIV5

Antitoxin

Source ID Structure
AlphaFold DB A0A3G9A886

References