Detailed information of TA system TA115385

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 21505..22058
• Replicon: plasmid pPagL15_3
• Accession: NZ_CP034151
• Organism: Pantoea agglomerans strain L15

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: CBF16_RS22775
• Protein ID: WP_031594618.1
• Coordinates: 21505..21819 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A7X2STP4
• Locus tag: CBF16_RS22780
• Protein ID: WP_033781302.1
• Coordinates: 21822..22058 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CBF16_RS22740	16994..17929	+	936	WP_124890869.1	hypothetical protein	-
CBF16_RS22745	18013..18462	+	450	WP_124890871.1	hypothetical protein	-
CBF16_RS22750	18560..19108	+	549	WP_124890873.1	hypothetical protein	-
CBF16_RS22755	19101..19895	+	795	WP_052272223.1	site-specific integrase	-
CBF16_RS22760	20214..20837	+	624	WP_031594621.1	AAA family ATPase	-
CBF16_RS22765	20834..21037	+	204	WP_031594620.1	hypothetical protein	-
CBF16_RS22770	21133..21363	+	231	WP_109653968.1	hypothetical protein	-
CBF16_RS22775	21505..21819	-	315	WP_031594618.1	CcdB family protein	Toxin
CBF16_RS22780	21822..22058	-	237	WP_033781302.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
CBF16_RS22785	22478..23752	-	1275	WP_033781347.1	translesion error-prone DNA polymerase V subunit UmuC	-
CBF16_RS22790	23761..24210	-	450	WP_124890875.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
CBF16_RS22795	24499..25026	+	528	WP_124890877.1	tail fiber assembly protein	-
CBF16_RS22800	25066..25824	-	759	WP_124890879.1	glycosyltransferase family 25 protein	-
CBF16_RS22805	26024..26201	+	178	Protein_26	Com family DNA-binding transcriptional regulator	-
CBF16_RS22810	26161..26871	+	711	WP_124890881.1	protein mom	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..66484	66484

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (9-78)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11658.60 Da
• Isoelectric Point: 5.8036

>T115385 WP_031594618.1 NZ_CP034151:c21819-21505 [Pantoea agglomerans]
MQFTVYSNTGKSAVYPLLLDVTSDIIGQLNRRIVIPLLPLEKYPSSTRPERLVPLIRLTDDKEYAVMTHEMASIPTRALG
AVFCDSSLYRTEIKAAIDFLLDGI

Nucleotide

• Length: 315 bp

>T115385 NZ_CP034151:c21819-21505 [Pantoea agglomerans]
ATGCAGTTCACTGTATACAGTAATACCGGGAAAAGCGCCGTCTATCCCCTGCTGCTCGATGTTACCAGCGATATTATCGG
GCAGTTGAACCGCAGGATTGTGATCCCCTTGCTGCCTCTTGAAAAGTACCCGAGCAGCACGCGACCTGAACGGCTGGTTC
CGCTTATCAGACTGACTGATGACAAAGAATATGCCGTAATGACGCATGAAATGGCGAGCATTCCCACTCGTGCTTTGGGG
GCTGTGTTCTGCGATTCATCTCTCTATCGTACAGAGATTAAAGCTGCTATTGATTTTCTTCTCGACGGTATCTAG

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8966.90 Da
• Isoelectric Point: 4.6234

>AT115385 WP_033781302.1 NZ_CP034151:c22058-21822 [Pantoea agglomerans]
MAITQRATQSVTMTVERDLLSRAREAGINLSATLTTALDAELRQYEAKKWQEENSEALEALNRFHDEHGCFSDEYRTF

Nucleotide

• Length: 237 bp

>AT115385 NZ_CP034151:c22058-21822 [Pantoea agglomerans]
ATGGCTATAACACAACGAGCAACGCAAAGCGTTACCATGACCGTAGAACGTGACTTACTCAGCCGTGCGCGTGAGGCCGG
AATTAATCTCAGTGCTACCCTGACCACGGCCCTCGACGCTGAACTTCGTCAGTACGAGGCAAAAAAATGGCAGGAGGAAA
ACAGCGAAGCACTGGAAGCTCTAAACCGCTTTCACGATGAACACGGTTGTTTCAGCGATGAGTACAGGACATTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A7X2STP4