Detailed information of TA system TA115376

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB(antitoxin)
• Location: 1891612..1892242
• Replicon: chromosome
• Accession: NZ_CP034148
• Organism: Pantoea agglomerans strain L15

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: CBF16_RS09040
• Protein ID: WP_122888909.1
• Coordinates: 1891612..1891794 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A349IF99
• Locus tag: CBF16_RS09045
• Protein ID: WP_010245317.1
• Coordinates: 1891817..1892242 (+)
• Length: 142 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CBF16_RS09020	1886956..1888353	+	1398	WP_010245332.1	class II fumarate hydratase	-
CBF16_RS09025	1888397..1889326	-	930	WP_124889943.1	DNA replication terminus site-binding protein	-
CBF16_RS09030	1889529..1890713	+	1185	WP_124889944.1	MFS transporter	-
CBF16_RS09035	1890921..1891415	+	495	WP_010245324.1	Rrf2 family transcriptional regulator	-
CBF16_RS09040	1891612..1891794	+	183	WP_122888909.1	type II toxin-antitoxin system HicA family toxin	Toxin
CBF16_RS09045	1891817..1892242	+	426	WP_010245317.1	hypothetical protein	Antitoxin
CBF16_RS09050	1892278..1892631	+	354	WP_124889945.1	type II toxin-antitoxin system HicB family antitoxin	-
CBF16_RS09055	1892717..1895869	-	3153	WP_124889946.1	multidrug efflux RND transporter permease subunit OqxB	-
CBF16_RS09060	1895882..1897036	-	1155	WP_010245312.1	efflux RND transporter periplasmic adaptor subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-60)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-82)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 7014.15 Da
• Isoelectric Point: 11.0643

>T115376 WP_122888909.1 NZ_CP034148:1891612-1891794 [Pantoea agglomerans]
MKYSEFRRWLIQQGAILTKHRSGSSHFKVFLGDRQSIFPDHGAKEMPEATRRKILKDLGL

Nucleotide

• Length: 183 bp

>T115376 NZ_CP034148:1891612-1891794 [Pantoea agglomerans]
ATGAAGTACAGTGAATTCAGACGCTGGCTCATTCAGCAGGGCGCTATTTTGACTAAGCACCGCAGTGGAAGCAGCCATTT
CAAGGTCTTCCTGGGCGACAGGCAATCCATTTTTCCGGATCACGGAGCAAAAGAAATGCCTGAAGCTACGCGCAGAAAAA
TTTTAAAAGATCTGGGCCTGTGA

Antitoxin

Protein

• Length: 142 a.a.
• Molecular weight: 15499.78 Da
• Isoelectric Point: 4.8263

>AT115376 WP_010245317.1 NZ_CP034148:1891817-1892242 [Pantoea agglomerans]
MFNYAVRLTHDAKTDTYEVSCRDLPLLHSVGDTVEEALAEAEDGLVTAISLEMDTRRPIPVASKPLEGEYIVSVPVLVAM
KAALYNAMIETGTRKVDLARQLGVNGPQVDRLLDVEHSSKVENVELALAQLGRKARIEIRT

Nucleotide

• Length: 426 bp

>AT115376 NZ_CP034148:1891817-1892242 [Pantoea agglomerans]
ATGTTTAATTATGCCGTCAGATTAACTCATGACGCCAAAACCGATACCTATGAGGTCTCGTGTCGTGATTTGCCGTTACT
GCATTCCGTCGGTGACACCGTTGAGGAAGCACTTGCAGAAGCCGAAGATGGACTGGTGACGGCAATCTCTCTCGAGATGG
ATACGCGTCGTCCGATTCCGGTTGCCAGTAAACCGCTGGAAGGAGAGTACATTGTCTCTGTGCCTGTACTGGTGGCAATG
AAAGCGGCACTTTACAACGCAATGATTGAAACCGGCACCCGTAAAGTCGATCTGGCACGTCAGCTGGGCGTGAATGGCCC
ACAGGTTGATCGACTACTGGATGTGGAGCACTCATCGAAAGTCGAAAATGTGGAGCTGGCGCTGGCTCAGCTGGGACGCA
AAGCCCGGATTGAAATTCGTACCTGA

Structures

Antitoxin

• AlphaFold DB: A0A349IF99