Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1003068..1003685 Replicon chromosome
Accession NZ_CP034148
Organism Pantoea agglomerans strain L15

Toxin (Protein)


Gene name Hha Uniprot ID E0LTU7
Locus tag CBF16_RS04640 Protein ID WP_003850458.1
Coordinates 1003068..1003283 (-) Length 72 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID E0LTU8
Locus tag CBF16_RS04645 Protein ID WP_003850455.1
Coordinates 1003308..1003685 (-) Length 126 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CBF16_RS04610 998986..999324 + 339 WP_003850469.1 P-II family nitrogen regulator -
CBF16_RS04615 999359..1000645 + 1287 WP_010255877.1 ammonium transporter AmtB -
CBF16_RS04620 1000698..1001561 - 864 WP_124889747.1 acyl-CoA thioesterase II -
CBF16_RS04625 1001752..1002309 + 558 WP_010255881.1 YbaY family lipoprotein -
CBF16_RS04630 1002349..1002660 - 312 WP_033756781.1 MGMT family protein -
CBF16_RS04640 1003068..1003283 - 216 WP_003850458.1 hemolysin expression modulator Hha Toxin
CBF16_RS04645 1003308..1003685 - 378 WP_003850455.1 Hha toxicity modulator TomB Antitoxin
CBF16_RS04650 1003833..1004186 - 354 WP_010255885.1 hypothetical protein -
CBF16_RS04655 1004528..1005196 + 669 WP_033766689.1 ABC transporter ATP-binding protein -
CBF16_RS04660 1005193..1006032 + 840 WP_062758242.1 metal ABC transporter permease -
CBF16_RS04665 1006048..1006926 + 879 WP_033785427.1 metal ABC transporter substrate-binding protein -
CBF16_RS04670 1006972..1007112 - 141 WP_010255896.1 type B 50S ribosomal protein L36 -
CBF16_RS04675 1007124..1007378 - 255 WP_010671219.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(12-68)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 72 a.a.        Molecular weight: 8510.90 Da        Isoelectric Point: 9.4825

>T115374 WP_003850458.1 NZ_CP034148:c1003283-1003068 [Pantoea agglomerans]
MNKTLTKTDYLMRLRRCRSLDTLERVIEKNKYELPEDELAVFYSAADHRLAELTMNKLYDKVPGSVWKFVR

Download         Length: 216 bp

>T115374 NZ_CP034148:c1003283-1003068 [Pantoea agglomerans]
ATGAACAAAACCCTGACTAAAACCGATTATTTGATGCGACTGCGGCGCTGCCGCTCTCTTGATACCCTGGAAAGGGTGAT
TGAAAAAAATAAGTATGAATTACCGGAAGATGAATTAGCGGTATTTTACTCAGCAGCTGACCATCGCCTGGCGGAACTGA
CGATGAACAAGCTCTACGATAAAGTCCCGGGTTCAGTATGGAAATTTGTCCGTTAA

Antitoxin


Download         Length: 126 a.a.        Molecular weight: 14636.25 Da        Isoelectric Point: 4.3976

>AT115374 WP_003850455.1 NZ_CP034148:c1003685-1003308 [Pantoea agglomerans]
MDEYSPKRHDIAQLKYLCESLFDDSMATLTDSHHGWVNDPTSESNLQLNDLIEHIASFTMNYKIKHVEDEALISQIDEYL
DDTFMLFSNYGVNSPDLQRWQRSAKRLFNLFTEECAFLQQPSHSL

Download         Length: 378 bp

>AT115374 NZ_CP034148:c1003685-1003308 [Pantoea agglomerans]
ATGGACGAATACTCACCGAAACGGCATGATATTGCCCAGCTTAAATACCTTTGTGAGAGTCTGTTTGACGACAGCATGGC
AACATTAACCGACAGTCATCATGGCTGGGTTAATGATCCCACTTCTGAAAGCAATCTACAGCTTAACGATTTGATTGAGC
ATATCGCCTCTTTCACCATGAATTACAAAATTAAGCACGTTGAAGATGAAGCGCTGATTTCGCAAATCGATGAATATCTT
GATGATACTTTTATGCTATTCAGCAATTATGGTGTCAATAGTCCGGATCTTCAGCGGTGGCAACGTTCAGCAAAACGCTT
ATTTAATCTGTTCACTGAAGAGTGCGCTTTTCTCCAGCAACCGAGCCATTCATTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1I5AGC3

Antitoxin

Source ID Structure
AlphaFold DB A0A1I5AG55

References