Detailed information of TA system TA115373

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 731878..732509
• Replicon: chromosome
• Accession: NZ_CP034148
• Organism: Pantoea agglomerans strain L15

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: CBF16_RS03350
• Protein ID: WP_071941068.1
• Coordinates: 731878..732057 (+)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A379AKM6
• Locus tag: CBF16_RS03355
• Protein ID: WP_033756573.1
• Coordinates: 732084..732509 (+)
• Length: 142 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CBF16_RS03340	728209..730110	+	1902	WP_124889695.1	pyruvate dehydrogenase complex dihydrolipoyllysine-residue acetyltransferase	-
CBF16_RS03345	730271..731695	+	1425	WP_010253950.1	dihydrolipoyl dehydrogenase	-
CBF16_RS03350	731878..732057	+	180	WP_071941068.1	type II toxin-antitoxin system HicA family toxin	Toxin
CBF16_RS03355	732084..732509	+	426	WP_033756573.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
CBF16_RS03360	732591..732731	+	141	WP_022625422.1	CRISPR-associated protein Cas1	-
CBF16_RS03365	732917..733846	+	930	WP_089414566.1	Rpn family recombination-promoting nuclease/putative transposase	-
CBF16_RS03370	734274..735173	-	900	WP_122459250.1	LysR family transcriptional regulator	-
CBF16_RS03375	735279..735788	+	510	WP_033780591.1	phenolic acid decarboxylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-58)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-128)

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6566.65 Da
• Isoelectric Point: 11.0668

>T115373 WP_071941068.1 NZ_CP034148:731878-732057 [Pantoea agglomerans]
MDSSTLISEMKADGWVLTRINGSHHHFTHPTKPGLVTVPHPKKDLPIGTVKSIRKQARI

Nucleotide

• Length: 180 bp

>T115373 NZ_CP034148:731878-732057 [Pantoea agglomerans]
ATGGATAGCAGCACGTTAATTTCCGAAATGAAGGCTGATGGCTGGGTTCTGACAAGGATTAACGGTAGCCATCACCATTT
CACACATCCGACAAAACCGGGGCTGGTTACAGTACCCCATCCTAAAAAAGACTTACCGATCGGTACGGTAAAGAGCATCA
GGAAACAAGCGAGAATTTAA

Antitoxin

Protein

• Length: 142 a.a.
• Molecular weight: 15436.62 Da
• Isoelectric Point: 4.7754

>AT115373 WP_033756573.1 NZ_CP034148:732084-732509 [Pantoea agglomerans]
MFYPIAIEAGDHEHAYGVIVPDLPGCFSAGDTLDEAIKNAKEAITGHIELCVELGHEIPAVSTIETLATNPEYQGYIWAL
VDVDVIRLLGGSEKINVTLPRSLIDRIDRCVASHPEYKSRSGFLAQVALERITAPINRPQK

Nucleotide

• Length: 426 bp

>AT115373 NZ_CP034148:732084-732509 [Pantoea agglomerans]
ATGTTTTATCCAATCGCGATTGAAGCGGGTGATCACGAACACGCATACGGCGTAATCGTTCCCGATTTACCAGGGTGTTT
CTCTGCCGGGGATACTCTTGATGAAGCCATTAAAAACGCCAAAGAGGCTATCACAGGGCATATCGAATTATGTGTAGAGC
TCGGTCATGAGATCCCTGCCGTGTCCACCATTGAGACTCTGGCCACGAATCCTGAATATCAGGGTTATATCTGGGCATTG
GTTGATGTAGATGTCATTCGTTTGCTGGGCGGCTCTGAAAAAATCAATGTCACTTTACCCAGGTCGTTAATTGATCGGAT
TGATCGTTGTGTGGCTTCACACCCGGAATACAAAAGTCGCTCGGGCTTTCTGGCTCAGGTAGCGCTTGAGCGTATTACTG
CTCCAATTAACAGGCCTCAAAAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A379AKM6