Detailed information of TA system TA115364

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemK-mazE/MazF(toxin)
• Location: 1524089..1524697
• Replicon: chromosome
• Accession: NZ_CP034141
• Organism: Paenibacillus sp. M-152

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: H6CFY1
• Locus tag: EGM68_RS06775
• Protein ID: WP_007429335.1
• Coordinates: 1524347..1524697 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: EGM68_RS06770
• Protein ID: WP_206099054.1
• Coordinates: 1524089..1524340 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGM68_RS06755	1520280..1520912	+	633	WP_058712320.1	LysE family transporter	-
EGM68_RS06760	1521023..1522300	+	1278	WP_019686558.1	outer membrane lipoprotein carrier protein LolA	-
EGM68_RS06765	1522569..1523765	+	1197	WP_129548889.1	alanine racemase	-
EGM68_RS06770	1524089..1524340	+	252	WP_206099054.1	ribbon-helix-helix protein, CopG family	Antitoxin
EGM68_RS06775	1524347..1524697	+	351	WP_007429335.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
EGM68_RS06780	1524876..1527092	+	2217	WP_206099045.1	helix-hairpin-helix domain-containing protein	-
EGM68_RS06785	1527197..1527331	-	135	WP_013369928.1	cortex morphogenetic protein CmpA	-
EGM68_RS06790	1527475..1527987	+	513	WP_013369929.1	SprT family protein	-
EGM68_RS06805	1528320..1529195	-	876	WP_129548892.1	Cof-type HAD-IIB family hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-36)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12822.77 Da
• Isoelectric Point: 5.6195

>T115364 WP_007429335.1 NZ_CP034141:1524347-1524697 [Paenibacillus sp. M-152]
MIVKRGDVFFADLSPVVGSEQGGVRPVLIIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAATHGFDRDSVVLLEQI
RTIDKQRLTDKITHLDEETMRKVSDSLQISLGLIDF

Nucleotide

• Length: 351 bp

>T115364 NZ_CP034141:1524347-1524697 [Paenibacillus sp. M-152]
TTGATCGTAAAGCGCGGCGACGTTTTTTTTGCAGACCTTTCACCCGTAGTCGGTTCCGAGCAAGGTGGCGTCAGACCTGT
GCTGATCATTCAAAATGATATCGGCAACCGGTTTAGCCCTACCGTGATCGTAGCAGCCATCACTGCACAGATTCAGAAGG
CAAAGCTGCCTACACACGTGGAAATCGACGCGGCGACGCATGGCTTTGATCGTGATTCTGTTGTTCTTCTGGAGCAAATC
CGGACAATCGACAAGCAAAGGCTTACCGACAAAATCACCCATTTGGATGAAGAAACCATGCGCAAAGTGAGCGACTCGCT
ACAGATCAGTCTCGGTTTGATTGATTTTTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9465.90 Da
• Isoelectric Point: 7.2022

>AT115364 WP_206099054.1 NZ_CP034141:1524089-1524340 [Paenibacillus sp. M-152]
MISLPDHLLKEVDGIVAMENSNRSEFIRQAMKLYVGERKKRYIREAMQRGYMEMAKINLTMASEAFHAEEDANSTLGRTV
SGV

Nucleotide

• Length: 252 bp

>AT115364 NZ_CP034141:1524089-1524340 [Paenibacillus sp. M-152]
ATGATCAGTTTGCCAGATCATCTCTTGAAGGAAGTGGACGGGATCGTAGCTATGGAAAATTCCAACCGCAGTGAATTTAT
CAGGCAGGCAATGAAGCTTTATGTAGGTGAACGTAAAAAACGTTACATTCGTGAAGCTATGCAGCGTGGATACATGGAAA
TGGCTAAAATTAACCTGACCATGGCATCCGAAGCCTTTCACGCGGAGGAAGATGCAAACAGCACCTTGGGCCGTACAGTT
AGCGGGGTGTAA

Structures

Toxin

• AlphaFold DB: H6CFY1