Detailed information of TA system TA115363

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 53688..54331
• Replicon: plasmid pG747_84.1Kb
• Accession: NZ_CP034140
• Organism: Klebsiella quasipneumoniae subsp. similipneumoniae strain G747

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: BME36_RS29915
• Protein ID: WP_087812770.1
• Coordinates: 53688..54104 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: BME36_RS29920
• Protein ID: WP_001261282.1
• Coordinates: 54101..54331 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BME36_RS29885	48998..49474	+	477	WP_000043177.1	DNA starvation/stationary phase protection protein	-
BME36_RS29890	49779..50129	-	351	WP_000928911.1	metalloregulator ArsR/SmtB family transcription factor	-
BME36_RS29895	50291..51949	+	1659	WP_000706606.1	FAD-dependent oxidoreductase	-
BME36_RS29900	52194..52658	+	465	WP_001243598.1	isoprenylcysteine carboxylmethyltransferase family protein	-
BME36_RS29905	52798..53500	+	703	Protein_55	IS6 family transposase	-
BME36_RS29915	53688..54104	-	417	WP_087812770.1	type II toxin-antitoxin system VapC family toxin	Toxin
BME36_RS29920	54101..54331	-	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
BME36_RS29925	54288..54700	+	413	Protein_58	hypothetical protein	-
BME36_RS29930	54872..55180	+	309	WP_048295720.1	hypothetical protein	-
BME36_RS29935	55216..55920	+	705	WP_087812771.1	hypothetical protein	-
BME36_RS29940	55920..56702	+	783	WP_032420986.1	site-specific integrase	-
BME36_RS29945	57327..58193	+	867	WP_032420987.1	replication initiation protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..84121	84121

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15187.58 Da
• Isoelectric Point: 6.4640

>T115363 WP_087812770.1 NZ_CP034140:c54104-53688 [Klebsiella quasipneumoniae subsp. similipneumoniae]
VNKIYMLDTNICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPDLVLEDWVR

Nucleotide

• Length: 417 bp

>T115363 NZ_CP034140:c54104-53688 [Klebsiella quasipneumoniae subsp. similipneumoniae]
GTGAACAAGATCTATATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCTGAAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAGTTTGAGCGGGTGCCGGATCTGGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT115363 WP_001261282.1 NZ_CP034140:c54331-54101 [Klebsiella quasipneumoniae subsp. similipneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT115363 NZ_CP034140:c54331-54101 [Klebsiella quasipneumoniae subsp. similipneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGAAGATTTAACCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GZG3