Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 120114..120850 Replicon plasmid pG747_218.9Kb
Accession NZ_CP034138
Organism Klebsiella quasipneumoniae subsp. similipneumoniae strain G747

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag BME36_RS28985 Protein ID WP_003026803.1
Coordinates 120114..120596 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag BME36_RS28990 Protein ID WP_003026799.1
Coordinates 120584..120850 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BME36_RS28965 115251..116705 - 1455 WP_071841200.1 EAL domain-containing protein -
BME36_RS28970 117308..117565 + 258 WP_023292103.1 hypothetical protein -
BME36_RS28975 117849..118481 + 633 WP_001567369.1 hypothetical protein -
BME36_RS28980 118510..119913 - 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
BME36_RS28985 120114..120596 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
BME36_RS28990 120584..120850 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
BME36_RS29000 121295..121525 - 231 WP_004206660.1 hypothetical protein -
BME36_RS29005 121539..121742 - 204 WP_004206661.1 hemolysin expression modulator Hha -
BME36_RS29010 121803..122294 - 492 WP_004206662.1 hypothetical protein -
BME36_RS29015 122428..123639 - 1212 WP_032415728.1 hypothetical protein -
BME36_RS29020 123636..123884 - 249 WP_004193994.1 hypothetical protein -
BME36_RS29025 124242..124592 + 351 WP_004206664.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
BME36_RS29030 124644..125006 + 363 WP_004206665.1 arsenite efflux transporter metallochaperone ArsD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - mrkJ / mrkF / mrkC / mrkB / mrkA 1..218944 218944
- flank IS/Tn - - 118510..119913 1403


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T115362 WP_003026803.1 NZ_CP034138:c120596-120114 [Klebsiella quasipneumoniae subsp. similipneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T115362 NZ_CP034138:c120596-120114 [Klebsiella quasipneumoniae subsp. similipneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGCAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT115362 WP_003026799.1 NZ_CP034138:c120850-120584 [Klebsiella quasipneumoniae subsp. similipneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT115362 NZ_CP034138:c120850-120584 [Klebsiella quasipneumoniae subsp. similipneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References