Detailed information of TA system TA115361

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-YafN
• Location: 25857..26379
• Replicon: plasmid pG747_218.9Kb
• Accession: NZ_CP034138
• Organism: Klebsiella quasipneumoniae subsp. similipneumoniae strain G747

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: A0A9J6S4Z8
• Locus tag: BME36_RS28490
• Protein ID: WP_004187019.1
• Coordinates: 25857..26141 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: A0A2J4ZSR4
• Locus tag: BME36_RS28495
• Protein ID: WP_004187025.1
• Coordinates: 26131..26379 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BME36_RS28465	21348..21776	+	429	WP_035897523.1	glutaredoxin-dependent arsenate reductase	-
BME36_RS28470	21903..23015	-	1113	WP_087812728.1	IS481-like element ISKpn28 family transposase	-
BME36_RS28475	23163..23951	+	789	Protein_31	ISNCY family transposase	-
BME36_RS28480	24040..25268	+	1229	WP_094081435.1	IS3 family transposase	-
BME36_RS28485	25281..25841	+	561	Protein_33	transposase	-
BME36_RS28490	25857..26141	-	285	WP_004187019.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BME36_RS28495	26131..26379	-	249	WP_004187025.1	plasmid stabilization protein	Antitoxin
BME36_RS28500	26754..27152	-	399	WP_049084174.1	helix-turn-helix domain-containing protein	-
BME36_RS28505	27201..28545	-	1345	Protein_37	ISNCY family transposase	-
BME36_RS28510	28665..29444	+	780	WP_176392677.1	hypothetical protein	-
BME36_RS28515	29438..30135	-	698	Protein_39	IS1 family transposase	-
BME36_RS28520	30347..31316	+	970	Protein_40	IS5-like element IS903B family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	mrkJ / mrkF / mrkC / mrkB / mrkA	1..218944	218944
	-	inside	IScluster/Tn	-	-	2983..31316	28333

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11003.72 Da
• Isoelectric Point: 10.5388

>T115361 WP_004187019.1 NZ_CP034138:c26141-25857 [Klebsiella quasipneumoniae subsp. similipneumoniae]
MTYKLAFNESALKEWKKLGHTLQVQFKKKLKERLENPRVPASQLHGRKDQYKIKLRGAGYRLVYRVEDDIVTVTVIGVGK
RENDDIYNATLNRN

Nucleotide

• Length: 285 bp

>T115361 NZ_CP034138:c26141-25857 [Klebsiella quasipneumoniae subsp. similipneumoniae]
ATGACCTATAAGCTGGCTTTCAACGAATCCGCTCTGAAAGAATGGAAGAAGCTGGGTCATACACTACAGGTACAGTTCAA
AAAGAAACTCAAAGAGCGCCTGGAGAACCCCAGAGTTCCGGCATCACAGCTACATGGACGTAAGGACCAGTATAAAATTA
AGCTACGTGGTGCCGGATATCGCCTTGTCTATCGTGTAGAGGACGACATTGTCACAGTCACTGTTATCGGCGTAGGCAAA
CGCGAAAACGACGACATTTATAATGCAACCCTTAATCGCAATTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9011.24 Da
• Isoelectric Point: 3.9947

>AT115361 WP_004187025.1 NZ_CP034138:c26379-26131 [Klebsiella quasipneumoniae subsp. similipneumoniae]
MAFQVLTTTAASITELKRDPMGTFHAGDGAPVAILNRNEPAFYCVPPALYAQLMELLEDEELGRMADERSGEKVIEVNID
DL

Nucleotide

• Length: 249 bp

>AT115361 NZ_CP034138:c26379-26131 [Klebsiella quasipneumoniae subsp. similipneumoniae]
ATGGCATTTCAGGTTCTGACCACTACAGCAGCTAGCATTACTGAACTTAAACGTGACCCTATGGGTACTTTCCACGCCGG
GGATGGCGCTCCGGTAGCCATTCTTAATCGTAATGAGCCTGCATTCTACTGTGTGCCACCGGCACTCTATGCCCAGTTGA
TGGAGCTTCTGGAAGATGAAGAGCTGGGACGGATGGCTGATGAGCGAAGCGGAGAAAAGGTTATAGAGGTCAATATTGAT
GACCTATAA

Structures

Antitoxin

• AlphaFold DB: A0A2J4ZSR4