Detailed information of TA system TA115360

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 79437..79962
• Replicon: plasmid pG747_150.8Kb
• Accession: NZ_CP034137
• Organism: Klebsiella quasipneumoniae subsp. similipneumoniae strain G747

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: J5VBT8
• Locus tag: BME36_RS27800
• Protein ID: WP_004197633.1
• Coordinates: 79657..79962 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A9E1GDP8
• Locus tag: BME36_RS27795
• Protein ID: WP_004197642.1
• Coordinates: 79437..79655 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BME36_RS27740	74543..75538	+	996	WP_019725651.1	hypothetical protein	-
BME36_RS27745	75611..75934	+	324	WP_019725650.1	hypothetical protein	-
BME36_RS30820	76368..76562	+	195	WP_019725033.1	hypothetical protein	-
BME36_RS27755	76606..76836	-	231	WP_019725034.1	hypothetical protein	-
BME36_RS27760	76850..77053	-	204	WP_019725040.1	hemolysin expression modulator Hha	-
BME36_RS27765	77087..77455	-	369	WP_015065502.1	hypothetical protein	-
BME36_RS27770	77499..77993	-	495	WP_009310053.1	hypothetical protein	-
BME36_RS27775	78024..78596	-	573	WP_015065504.1	hypothetical protein	-
BME36_RS27780	78593..78841	-	249	WP_019725036.1	hypothetical protein	-
BME36_RS27795	79437..79655	+	219	WP_004197642.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
BME36_RS27800	79657..79962	+	306	WP_004197633.1	type II toxin-antitoxin system toxin CcdB	Toxin
BME36_RS27805	80131..80532	+	402	WP_072199347.1	hypothetical protein	-
BME36_RS27810	80559..80882	+	324	WP_004197641.1	hypothetical protein	-
BME36_RS27815	80879..81895	+	1017	WP_004197639.1	hypothetical protein	-
BME36_RS27820	82093..82887	+	795	WP_004197635.1	site-specific integrase	-
BME36_RS27825	83351..83530	-	180	WP_006788217.1	hypothetical protein	-
BME36_RS27830	83650..84276	-	627	WP_004197649.1	ParA family plasmid-partitioning AAA ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / blaCTX-M-15 / dfrA14 / qnrB1 / tet(A) / aac(6')-Ib-cr / blaOXA-1 / aac(3)-IIa	-	1..150833	150833

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11585.27 Da
• Isoelectric Point: 6.4661

>T115360 WP_004197633.1 NZ_CP034137:79657-79962 [Klebsiella quasipneumoniae subsp. similipneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T115360 NZ_CP034137:79657-79962 [Klebsiella quasipneumoniae subsp. similipneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGACAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGGCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8376.34 Da
• Isoelectric Point: 4.7405

>AT115360 WP_004197642.1 NZ_CP034137:79437-79655 [Klebsiella quasipneumoniae subsp. similipneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT115360 NZ_CP034137:79437-79655 [Klebsiella quasipneumoniae subsp. similipneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A2A5MCG5