Detailed information of TA system TA115358

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 4774326..4775029
• Replicon: chromosome
• Accession: NZ_CP034136
• Organism: Klebsiella quasipneumoniae subsp. similipneumoniae strain G747

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: -
• Locus tag: BME36_RS24185
• Protein ID: WP_064190208.1
• Coordinates: 4774326..4774667 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A2Z3WZC0
• Locus tag: BME36_RS24190
• Protein ID: WP_032410777.1
• Coordinates: 4774688..4775029 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BME36_RS24170	4771059..4771697	-	639	WP_064190206.1	HNH endonuclease	-
BME36_RS24175	4771877..4772746	+	870	WP_064190207.1	HNH endonuclease	-
BME36_RS24180	4773064..4774071	-	1008	WP_004108748.1	restriction endonuclease	-
BME36_RS24185	4774326..4774667	-	342	WP_064190208.1	TA system toxin CbtA family protein	Toxin
BME36_RS24190	4774688..4775029	-	342	WP_032410777.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
BME36_RS24195	4775040..4775582	-	543	WP_064190209.1	DNA repair protein RadC	-
BME36_RS24200	4775595..4776037	-	443	Protein_4659	antirestriction protein	-
BME36_RS24205	4776068..4776889	-	822	WP_064190210.1	DUF945 domain-containing protein	-
BME36_RS24210	4777009..4777482	-	474	WP_064190211.1	hypothetical protein	-
BME36_RS24215	4777554..4778006	-	453	WP_077268172.1	hypothetical protein	-
BME36_RS24220	4778042..4778758	-	717	WP_064190213.1	WYL domain-containing protein	-
BME36_RS24225	4779002..4779877	-	876	WP_064190214.1	GTPase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4774326..4789382	15056

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-109)

Antitoxin

• Antitoxin CbeA (13-111)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12709.70 Da
• Isoelectric Point: 8.5104

>T115358 WP_064190208.1 NZ_CP034136:c4774667-4774326 [Klebsiella quasipneumoniae subsp. similipneumoniae]
MKTLPATTPQAAKLCLSPVSVWQMLLACLLEQHYGLTLNDTPFSDETVIQEHIDAGISLADAVNFLVEKYGLVRIDRRGF
NWQEQSPYLRAVDILRARQATGLLKRNRISAAR

Nucleotide

• Length: 342 bp

>T115358 NZ_CP034136:c4774667-4774326 [Klebsiella quasipneumoniae subsp. similipneumoniae]
ATGAAAACTCTACCTGCAACAACTCCGCAGGCGGCGAAGCTATGTCTGTCGCCCGTGTCTGTCTGGCAAATGCTGCTTGC
ATGCCTGCTGGAACAACATTATGGCCTGACACTCAACGACACGCCGTTCAGTGATGAAACGGTAATTCAGGAACATATCG
ATGCCGGTATATCTTTGGCCGATGCCGTTAATTTTCTGGTGGAAAAGTACGGGCTGGTTCGTATTGATCGCAGGGGATTC
AACTGGCAAGAACAGTCCCCATATCTGCGGGCGGTCGATATTCTGCGGGCGCGACAGGCAACTGGTTTATTGAAAAGAAA
TCGTATTAGCGCGGCACGGTGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12765.40 Da
• Isoelectric Point: 7.0269

>AT115358 WP_032410777.1 NZ_CP034136:c4775029-4774688 [Klebsiella quasipneumoniae subsp. similipneumoniae]
MKSTDSENDSFLKWGLNRNVTPCFGARLVQEGNRLHYLADRASITGKFSEAECLKLDVAFPHFISQMESMLTTGEMNPRH
AHRVTLYHNGFTCEADTLGSCGYVYIAVYSTHR

Nucleotide

• Length: 342 bp

>AT115358 NZ_CP034136:c4775029-4774688 [Klebsiella quasipneumoniae subsp. similipneumoniae]
ATGAAATCTACTGACTCAGAGAACGATTCATTTCTAAAATGGGGACTGAATCGTAATGTGACACCTTGCTTTGGCGCTCG
TCTGGTACAGGAGGGAAACCGGCTGCACTATCTGGCTGACCGGGCCAGTATCACCGGTAAGTTCAGCGAAGCAGAATGTC
TTAAGCTGGATGTGGCATTCCCGCATTTTATCAGCCAGATGGAATCGATGCTGACTACTGGTGAAATGAATCCTCGCCAT
GCCCACCGCGTCACCCTGTACCACAATGGTTTCACCTGCGAAGCCGATACCCTTGGCAGTTGCGGCTACGTATACATCGC
CGTTTACTCCACCCATCGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A2Z3WZC0