Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4111091..4111710 Replicon chromosome
Accession NZ_CP034136
Organism Klebsiella quasipneumoniae subsp. similipneumoniae strain G747

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag BME36_RS20940 Protein ID WP_002892050.1
Coordinates 4111492..4111710 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag BME36_RS20935 Protein ID WP_002892066.1
Coordinates 4111091..4111465 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BME36_RS20925 4106244..4107437 + 1194 WP_004204747.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
BME36_RS20930 4107460..4110606 + 3147 WP_004204748.1 multidrug efflux RND transporter permease subunit -
BME36_RS20935 4111091..4111465 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
BME36_RS20940 4111492..4111710 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
BME36_RS20945 4111868..4112434 + 567 WP_004204750.1 maltose O-acetyltransferase -
BME36_RS30655 4112406..4112528 - 123 WP_032426076.1 hypothetical protein -
BME36_RS20950 4112571..4113041 + 471 WP_049093338.1 YlaC family protein -
BME36_RS20955 4113010..4114467 - 1458 WP_049117056.1 PLP-dependent aminotransferase family protein -
BME36_RS20960 4114568..4115263 + 696 Protein_4029 GNAT family N-acetyltransferase -
BME36_RS20965 4115263..4115403 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
BME36_RS20970 4115403..4115666 - 264 WP_004204754.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T115357 WP_002892050.1 NZ_CP034136:4111492-4111710 [Klebsiella quasipneumoniae subsp. similipneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T115357 NZ_CP034136:4111492-4111710 [Klebsiella quasipneumoniae subsp. similipneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAACAAATATGAACTTTCTGATAATGAGCTGGCGGTATTTTACTCAGCCGCCGATCATCGTCTGGCTGAAC
TGACTATGAATAAGCTGTACGATAAAATCCCGACGTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT115357 WP_002892066.1 NZ_CP034136:4111091-4111465 [Klebsiella quasipneumoniae subsp. similipneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT115357 NZ_CP034136:4111091-4111465 [Klebsiella quasipneumoniae subsp. similipneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATCGCACAACTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACATCGGCTGTCAATCTCCAGTTAAATGAACTGATCGAGC
ACATTGCCACATTCGCCCTTAATTATAAAATTAAGTACGCTGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACATTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAATCGGTT
ATTTCGTTGTTTTGTCAACGCCAGCCGGGAAAATCCGGCCAGTCTTTCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References